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Titolo:
150-kDa oxygen-regulated protein (ORP150) functions as a novel molecular chaperone in MDCK cells
Autore:
Bando, Y; Ogawa, S; Yamauchi, A; Kuwabara, K; Ozawa, K; Hori, O; Yanagi, H; Tamatani, M; Tohyama, M;
Indirizzi:
Osaka Univ, Grad Sch Med, Dept Anat & Neurosci, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 eurosci, Suita, Osaka 5650871, Japan Osaka Univ, Grad Sch Med, Dept Med 1, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 t Med 1, Suita, Osaka 5650871, Japan Osaka Rosai Hosp, Dept Med, Div Nephrol, Osaka 5918025, Japan Osaka Rosai Hosp Osaka Japan 5918025 , Div Nephrol, Osaka 5918025, Japan HSP Res Inst, Shimogyo Ku, Kyoto 6008813, Japan HSP Res Inst Kyoto Japan6008813 Inst, Shimogyo Ku, Kyoto 6008813, Japan Japan Sci & Technol, Core Res Evolut Sci & Technol, Kawaguchi 3320012, Japan Japan Sci & Technol Kawaguchi Japan 3320012 ol, Kawaguchi 3320012, Japan
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 6, volume: 278, anno: 2000,
pagine: C1172 - C1182
SICI:
0363-6143(200006)278:6<C1172:1OP(FA>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOPLASMIC-RETICULUM PROTEIN; MONONUCLEAR PHAGOCYTES; GLYCOPROTEIN COMPLEX; DISULFIDE ISOMERASE; STRESS PROTEINS; EXPRESSION; HYPOXIA; KIDNEY; IMMUNOGLOBULIN; INDUCTION;
Keywords:
hypoxia; energy metabolism; renal epithelium; ischemia; adenosine 5 '-triphosphate kinetics;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Bando, Y Osaka Univ, Grad Sch Med, Dept Anat & Neurosci, 2-2 Yamadaoka, Suita, Osaka 5650871, Japan Osaka Univ 2-2 Yamadaoka Suita Osaka Japan 5650871 5650871, Japan
Citazione:
Y. Bando et al., "150-kDa oxygen-regulated protein (ORP150) functions as a novel molecular chaperone in MDCK cells", AM J P-CELL, 278(6), 2000, pp. C1172-C1182

Abstract

To assess the participation of the 150-kDa oxygen-regulated protein (ORP150) in protein transport, its function in Madin-Darby canine kidney (MDCK) cells was studied. Exposure of MDCK cells to hypoxia resulted in an increaseof ORP150 antigen and increased binding of ORP150 to GP80/clusterin (80-kDa glycoprotein), a natural secretory protein in this cell line. In ORP150 antisense transformant MDCK cells, GP80 was retained within the endoplasmic reticulum after exposure to hypoxia. Metabolic labeling showed the delay ofGP80 maturation in antisense transformants in hypoxia, whereas its maturedform was detected in wild-type cells, indicating a role of ORP150 in protein transport, especially in hypoxia. The affinity chromatographic analysis of ORP150 suggested its ability to bind to ATP-agarose. Furthermore, the ATP hydrolysis analysis showed that ORP150 can release GP80 at a lower ATP concentration. These data indicate that ORP150 may function as a unique molecular chaperone in renal epithelial cells by facilitating protein transport/maturation in an environment where less ATP is accessible.

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Documento generato il 29/10/20 alle ore 20:46:44