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Titolo:
Carrier-mediated uptake of cisplatin by the OK renal epithelial cell line
Autore:
Endo, T; Kimura, O; Sakata, M;
Indirizzi:
Hlth Sci Univ Hokkaido, Fac Pharmaceut Sci, Ishikari, Hokkaido 0610293, Japan Hlth Sci Univ Hokkaido Ishikari Hokkaido Japan 0610293 ido 0610293, Japan
Titolo Testata:
TOXICOLOGY
fascicolo: 2-3, volume: 146, anno: 2000,
pagine: 187 - 195
SICI:
0300-483X(20000505)146:2-3<187:CUOCBT>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
LLC-PK1 CELLS; ORGANIC-ANIONS; NEPHROTOXICITY; TRANSPORT; CADMIUM; CATIONS; KIDNEY; CIS-DIAMMINEDICHLOROPLATINUM(II); ACCUMULATION; CARBOPLATIN;
Keywords:
cisplatin; OK cells; organic cation transporter; tetraethylammonium; apical membrane; basolateral membrane;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Endo, T Hlth Sci Univ Hokkaido, Fac Pharmaceut Sci, 1757 Ishikari-Tobetsu,Ishikari, Hokkaido 0610293, Japan Hlth Sci Univ Hokkaido 1757 Ishikari-Tobetsu Ishikari Hokkaido Japan 0610293
Citazione:
T. Endo et al., "Carrier-mediated uptake of cisplatin by the OK renal epithelial cell line", TOXICOLOGY, 146(2-3), 2000, pp. 187-195

Abstract

The purpose of this study was to investigate whether transport of cis-diamminedichloroplatinum II (cisplatin) across renal epithelial cell line OK cells is mediated by the organic cation transport system. OK cell monolayers cultured on permeable membranes were incubated with 100 mu M cisplatin on the apical or basolateral side, and the cellular accumulation and the transport of cisplatin across the monolayer were measured. The accumulation from the basolateral medium and the basolateral-to-apical transport of cisplatinwere higher than the accumulation from the apical medium and the apical-to-basolateral transport, respectively. The cell monolayers were incubated with different concentrations of cisplatin (0.02 similar to 3 mM) in the basolateral medium. The relationship between the cisplatin concentrations in the medium and in the cells revealed that cisplatin accumulation tended to besaturable. The basolateral-to-apical transport of cisplatin was increased when the pH of the apical medium was decreased, with a concomitant decreasein the accumulation of cisplatin. Coincubation of cisplatin with tetraethylammonium (TEA), a typical substrate for the organic cation transporter, significantly decreased the accumulation and transport of cisplatin From the basolateral medium. These results suggest that the uptake and basolateral-to-apical transport of cisplatin are mediated by not only simple diffusion but also by the organic cation transport system. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 04:16:18