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Titolo:
Use of sonication for measuring acid phosphatase activity in soil
Autore:
De Cesare, F; Garzillo, AMV; Buonocore, V; Badalucco, L;
Indirizzi:
Univ Tuscia, Dipartimento Agrobiol & Agrochim, I-01100 Viterbo, Italy UnivTuscia Viterbo Italy I-01100 iol & Agrochim, I-01100 Viterbo, Italy Univ Palermo, Dipartimento Ingn & Tecnol AgroForestali, I-90128 Palermo, Italy Univ Palermo Palermo Italy I-90128 AgroForestali, I-90128 Palermo, Italy
Titolo Testata:
SOIL BIOLOGY & BIOCHEMISTRY
fascicolo: 6, volume: 32, anno: 2000,
pagine: 825 - 832
SICI:
0038-0717(200006)32:6<825:UOSFMA>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
ORGANIC-MATTER; ENZYME-ACTIVITY; SIZE FRACTIONS; EXTRACTION; ATP; DISPERSION; BACTERIA;
Keywords:
soil acid phosphatase; soil enzyme assay; sonication;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: De Cesare, F Univ Tuscia, Dipartimento Agrobiol & Agrochim, Via S Camillo Lellis, I-01100 Viterbo, Italy Univ Tuscia Via S Camillo Lellis Viterbo Italy I-01100 Italy
Citazione:
F. De Cesare et al., "Use of sonication for measuring acid phosphatase activity in soil", SOIL BIOL B, 32(6), 2000, pp. 825-832

Abstract

Extracellular enzymes in soil often occur in immobilised forms, a state that may alter their interactions with substrates in comparison with enzymes in the solution phase. Sonication was evaluated for its usefulness in studying immobilised acid phosphatase by dispersing soil aggregates. Factors affecting soil dispersion during ultrasound application were soil extraction ratio, total applied energy and power output ml(-1) of sonicated soil slurry. For the clay loam soil used, optimal values for these variables were, respectively, 1:6 (w/v) and, at least, 1800 J ml(-1) and 15 W ml(-1). At the optimal sonication conditions for soil dispersion a substantial increase in phosphatase activity (up to 156% greater than the non-sonicated control) was induced by sonication. This increase in activity with sonication, which coincided with a release of soil chromophores, might be related to the exposure or release from aggregates of the extracellular enzyme fraction immobilised on humic colloids. Analysis of multiple regression between the phosphatase activity (dependent variable) and chromophore solubilisation and ATP release (independent variables) suggested the increased activity was from complexed enzymes that were released and not due to cell lysis. Soil treatment with sonication appeared to have liberated a large dormant portion of acid phosphatase activity. Coefficients of variation of the activity decreasedgreatly (from 20% in control soil to 4% as an average after sonication). (C) 2000 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 21:23:14