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Titolo:
Maximizing RNA folding rates: A balancing act
Autore:
Thirumalai, D; Woodson, SA;
Indirizzi:
Univ Maryland, Dept Chem, College Pk, MD 20742 USA Univ Maryland College Pk MD USA 20742 Dept Chem, College Pk, MD 20742 USA Univ Maryland, Inst Phys Sci & Technol, College Pk, MD 20742 USA Univ Maryland College Pk MD USA 20742 & Technol, College Pk, MD 20742 USA Johns Hopkins Univ, TC Jenkins Dept Biophys, Baltimore, MD 21218 USA JohnsHopkins Univ Baltimore MD USA 21218 iophys, Baltimore, MD 21218 USA
Titolo Testata:
RNA-A PUBLICATION OF THE RNA SOCIETY
fascicolo: 6, volume: 6, anno: 2000,
pagine: 790 - 794
SICI:
1355-8382(200006)6:6<790:MRFRAB>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
SELF-SPLICING RNA; GROUP-I RIBOZYME; TETRAHYMENA RIBOZYME; TERTIARY STRUCTURE; CATALYTIC CORE; RIBONUCLEASE-P; KINETIC TRAPS; DOMAIN; PATHWAYS; THERMODYNAMICS;
Keywords:
group I intron; ribozyme; RNA folding; RNA structure;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Thirumalai, D Univ Maryland, Dept Chem, College Pk, MD 20742 USA Univ Maryland College Pk MD USA 20742 lege Pk, MD 20742 USA
Citazione:
D. Thirumalai e S.A. Woodson, "Maximizing RNA folding rates: A balancing act", RNA, 6(6), 2000, pp. 790-794

Abstract

Large ribozymes typically require very long times to refold into their active conformation in vitro, because the RNA is easily trapped in metastable misfolded structures. Theoretical models show that the probability of misfolding is reduced when local and long-range interactions in the RNA are balanced. Using the folding kinetics of the Tetrahymena ribozyme as an example,we propose that folding rates are maximized when the free energies of forming independent domains are similar to each other. A prediction is that thefolding pathway of the ribozyme can be reversed by inverting the relative stability of the tertiary domains. This result suggests strategies for optimizing ribozyme sequences for therapeutics and structural studies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 01:23:47