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Titolo:
Plant regeneration from hairy roots induced by infection with Agrobacterium rhizogenes in Crotalaria juncea L.
Autore:
Ohara, A; Akasaka, Y; Daimon, H; Mii, M;
Indirizzi:
Chiba Univ, Fac Hort, Chiba 2718510, Japan Chiba Univ Chiba Japan 2718510 hiba Univ, Fac Hort, Chiba 2718510, Japan Hokkaido Natl Agr Expt Stn, Kasai, Hokkaido 0820071, Japan Hokkaido Natl Agr Expt Stn Kasai Hokkaido Japan 0820071 do 0820071, Japan Univ Osaka Prefecture, Coll Agr, Osaka 5998531, Japan Univ Osaka Prefecture Osaka Japan 5998531 Coll Agr, Osaka 5998531, Japan
Titolo Testata:
PLANT CELL REPORTS
fascicolo: 6, volume: 19, anno: 2000,
pagine: 563 - 568
SICI:
0721-7714(200005)19:6<563:PRFHRI>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
FERTILE TRANSGENIC PLANTS; CROWN GALL; TRANSFORMATION; INVITRO; DNA; VITRIFICATION; MIKIMOPINE;
Keywords:
Agrobacterium rhizogenes; Crotalaria juncea; plant regeneration; transformation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Mii, M Chiba Univ, Fac Hort, Chiba 2718510, Japan Chiba Univ Chiba Japan2718510 iv, Fac Hort, Chiba 2718510, Japan
Citazione:
A. Ohara et al., "Plant regeneration from hairy roots induced by infection with Agrobacterium rhizogenes in Crotalaria juncea L.", PL CELL REP, 19(6), 2000, pp. 563-568

Abstract

Hairy roots were induced from leaf segments of Crotalaria juncea, which isused as a green manure crop antagonistic to nematodes, by infection with amikimopine type wild strain of Agrobacterium rhizogenes A13 (MAFF02-10266). These roots exhibited vigorous growth and abundant lateral branching on half-strength Murashige and Skoog (1/2MS) medium without phytohormones. The adventitious shoots were induced from 30% of root segments 3 months after transfer onto medium containing 3 mg/l benzyl adenine. These shoots producedroots 1 month after transfer onto 1.2% agar-solidified 1/2MS medium without phytohormones. Regenerated plants were successfully grown under greenhouse conditions. The transgenic nature of the regenerated plants was confirmedby Southern-blot analysis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 13:25:47