Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Calpain-mediated degradation of PSD-95 in developing and adult rat brain
Autore:
Lu, XY; Rong, YQ; Baudry, M;
Indirizzi:
Univ So Calif, Neurosci Program, Los Angeles, CA 90089 USA Univ So Calif Los Angeles CA USA 90089 Program, Los Angeles, CA 90089 USA
Titolo Testata:
NEUROSCIENCE LETTERS
fascicolo: 2, volume: 286, anno: 2000,
pagine: 149 - 153
SICI:
0304-3940(20000602)286:2<149:CDOPID>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
DENSITY PROTEIN PSD-95; CHANNELS; FAMILY; DOMAIN;
Keywords:
PSD-95; calpain; spines; hippocampus; development; synaptic plasticity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Baudry, M Univ So Calif, Neurosci Program, HNB 124, Los Angeles, CA 90089 USA Univ So Calif HNB 124 Los Angeles CA USA 90089 les, CA 90089 USA
Citazione:
X.Y. Lu et al., "Calpain-mediated degradation of PSD-95 in developing and adult rat brain", NEUROSCI L, 286(2), 2000, pp. 149-153

Abstract

PSD-95 is a major postsynaptic density protein that is degraded as a result of synaptic activity. We used four different methods to test the hypothesis that calpain is involved in PSD-95 turnover. Treatment of synaptic membranes with purified calpain resulted in a decrease in immunoreactivity of the native 95 kDa protein and the appearance of two smaller molecular weight species, migrating at 50 and 36 kDa, respectively. Calcium treatment of frozen-thawed brain sections produced an identical digestion pattern, an effect blocked by calpain inhibitors. N-methyl-D-aspartate treatment of organotypic hippocampal cultures produced truncation of PSD-95 and accumulation of the 36 kDa species. Finally, calpain-generated degradation products of PSD95 were prominent in neonatal hippocampus, and disappeared with postnatal development. Our data suggest that PSD-95 is a substrate for calpain, and that calpain-mediated truncation contributes to PSD-95 turnover. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 12:27:54