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Titolo:
Analysis of the role of egg integrins in sperm-egg binding and fusion
Autore:
Takahashi, Y; Yamakawa, N; Matsumoto, K; Toyoda, Y; Furukawa, K; Sato, E;
Indirizzi:
Tokyo Metropolitan Inst Gerontol, Dept Biosignal Res, Itabashi Ku, Tokyo 1730015, Japan Tokyo Metropolitan Inst Gerontol Tokyo Japan 1730015 okyo 1730015, Japan Univ Tokyo, Inst Med Sci, Dept Reprod & Dev Biol, Tokyo, Japan Univ TokyoTokyo Japan st Med Sci, Dept Reprod & Dev Biol, Tokyo, Japan
Titolo Testata:
MOLECULAR REPRODUCTION AND DEVELOPMENT
fascicolo: 3, volume: 56, anno: 2000,
pagine: 412 - 423
SICI:
1040-452X(200007)56:3<412:AOTROE>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
ZONA-FREE MOUSE; FERTILIN-BETA; MONOCLONAL-ANTIBODIES; PLASMA-MEMBRANE; INTRASPLENIC IMMUNIZATION; DISINTEGRIN DOMAIN; HAMSTER EGGS; IN-VITRO; RECEPTOR; OOCYTES;
Keywords:
sperm receptor; integrin alpha 6 beta 1; mouse;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Yamakawa, N Tokyo Metropolitan Inst Gerontol, Dept Biosignal Res, ItabashiKu, 35-2 Sakae Cho, Tokyo 1730015, Japan Tokyo Metropolitan Inst Gerontol 35-2 Sakae Cho Tokyo Japan 1730015
Citazione:
Y. Takahashi et al., "Analysis of the role of egg integrins in sperm-egg binding and fusion", MOL REPROD, 56(3), 2000, pp. 412-423

Abstract

Sperm-egg fusion is believed to be mediated via specific molecular interactions. Integrin alpha 6 beta 1 is a strong candidate for a sperm receptor on the egg plasma membrane. However, the ability of the egg integrin alpha 6beta 1 to interact with molecules on intact sperm has not yet been proven. In this report, possible involvement of integrin alpha 6 beta 1 in sperm-egg interactions was examined by biochemical and immunocytochemical analyses. To identify egg molecules that specifically interact with sperm, we firstincubated sperm with biotin-labeled egg surface proteins. Under this condition, solubilized proteins from eggs inhibited sperm-egg fusion. Western blot analysis under reducing conditions indicated that a major-labeled band of 135 kDa bound to sperm. An immunodepletion experiment using the anti-integrin alpha 6 antibody GoH3 indicated that the 135 kDa egg surface molecule that bound to sperm was the integrin alpha 6 subunit. To investigate the potential involvement of integrin alpha 6 beta 1 in sperm-egg fusion, we nextexamined the localization of integrin a6 and beta 1 subunits before and after fertilization by confocal laser microscopy. At an early stage of sperm-egg fusion, the integrin alpha 6 and beta 1 subunits were accumulated at the sperm binding site. The frequency of cluster formation was closely related to that of sperm-egg fusion, indicating that integrin receptors are accumulated by sperm destined for fusion. Taken together, these results stronglysuggest that the integrin alpha 6 beta 1 is involved in sperm-egg binding leading to fusion via direct association of the integrin alpha 6 with sperm. Mol. Reprod. Dev. 56:412-423, 2000. (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/01/21 alle ore 17:11:08