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Titolo:
PDX-1 and cell-cell contact act in synergy to promote delta-cell development in a human pancreatic endocrine precursor cell line
Autore:
Itkin-Ansari, P; Demeterco, C; Bossie, S; de la Tour, DD; Beattie, GM; Movassat, J; Mally, MI; Hayek, A; Levine, F;
Indirizzi:
Univ Calif San Diego, Sch Med, Ctr Mol Genet, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 Genet, La Jolla, CA 92093 USA Univ Calif San Diego, Whittier Inst Diabet & Endocrinol, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 crinol, La Jolla, CA 92093 USA
Titolo Testata:
MOLECULAR ENDOCRINOLOGY
fascicolo: 6, volume: 14, anno: 2000,
pagine: 814 - 822
SICI:
0888-8809(200006)14:6<814:PACCAI>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
INSULIN GENE-TRANSCRIPTION; HUMAN FETAL PANCREAS; BETA-CELLS; HOMEOBOX FACTOR; ISLET CELLS; COOPERATIVE INTERACTIONS; RETROVIRAL VECTORS; SOMATOSTATIN GENE; EPITHELIAL-CELLS; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Levine, F Univ Calif San Diego, Sch Med, Ctr Mol Genet, La Jolla, CA 92093USA Univ Calif San Diego La Jolla CA USA 92093 Jolla, CA 92093 USA
Citazione:
P. Itkin-Ansari et al., "PDX-1 and cell-cell contact act in synergy to promote delta-cell development in a human pancreatic endocrine precursor cell line", MOL ENDOCR, 14(6), 2000, pp. 814-822

Abstract

Cell lines from the fetal and adult pancreas that were developed by retroviral transfer of the SV40T and ras(val12) oncogenes lose insulin expressionbut retain extremely low levels of somatostatin and glucagon mRNA. In contrast to expanded populations of primary human islet cells, none of them express the homeodomain transcription factor PDX-1. When that factor was expressed in the cell lines by retroviral-mediated gene transfer, one of the cell lines, TRM-6, derived from human fetal islets, exhibited a 10- to 100-fold increase in somatostatin gene expression. This is the first report of induction of the endogenous somatostatin gene by PDX-1, Promotion of cell-cellcontact by aggregation of TRM-6/PDX-1 into islet-like clusters produced a further 10- to 100-fold increase in somatostatin mRNA, to a level similar to that of freshly isolated islets, which resulted in production of somatostatin protein. Thus, we demonstrate here that signals induced by cell-cell contact act in synergy with PDX-1 to up-regulate the endogenous somatostatinpromoter in an immortalized cell line from human fetal islets. This systemprovides a powerful model for studying human islet cell development and, particularly, the role of cell-cell contact in the differentiation process.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 05:12:32