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Titolo:
Autonomous multi-lineage differentiation in vitro of primitive CD34(+) cells from patients with chronic myeloid leukemia
Autore:
Jiang, X; Fujisaki, T; Nicolini, F; Berger, M; Holyoake, T; Eisterer, W; Eaves, C; Eaves, A;
Indirizzi:
British Columbia Canc Agcy, Terry Fox Lab, Vancouver, BC V5Z 1L3, Canada British Columbia Canc Agcy Vancouver BC Canada V5Z 1L3 BC V5Z 1L3, Canada Univ British Columbia, Dept Med Genet, Vancouver, BC V5Z 1M9, Canada Univ British Columbia Vancouver BC Canada V5Z 1M9 ver, BC V5Z 1M9, Canada Univ British Columbia, Dept Med & Expt Pathol & Lab Med, Vancouver, BC V5Z1M9, Canada Univ British Columbia Vancouver BC Canada V5Z 1M9 uver, BC V5Z1M9, Canada
Titolo Testata:
LEUKEMIA
fascicolo: 6, volume: 14, anno: 2000,
pagine: 1112 - 1121
SICI:
0887-6924(200006)14:6<1112:AMDIVO>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHRONIC MYELOGENOUS LEUKEMIA; COLONY-STIMULATING FACTOR; CHRONIC GRANULOCYTIC-LEUKEMIA; HUMAN HEMATOPOIETIC-CELLS; FORMING CELLS; MEGAKARYOCYTE PROGENITORS; SUPPLEMENTED CULTURES; STEEL FACTOR; IN-VITRO; BCR-ABL;
Keywords:
autocrine; hematopoiesis; progenitors; megakaryocytes; IL-3; CML;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Eaves, A British Columbia Canc Agcy, Terry Fox Lab, 601 W 10th Ave, Vancouver, BC V5Z 1L3, Canada British Columbia Canc Agcy 601 W 10th Ave VancouverBC Canada V5Z 1L3
Citazione:
X. Jiang et al., "Autonomous multi-lineage differentiation in vitro of primitive CD34(+) cells from patients with chronic myeloid leukemia", LEUKEMIA, 14(6), 2000, pp. 1112-1121

Abstract

Neoplastic CD34(+) cells from chronic myeloid leukemia (CML) patients proliferate in vitro in the absence of serum or defined growth factors due to an autocrine mechanism involving IL-3 and G-CSF (Jiang et ai. Proc Natl AcadSci USA 1999; 96: 12804). Detailed examination of the various cell types produced in such cultures has now demonstrated the rapid, factor-independent, generation of clonogenic progenitors for all lineages (granulocyte-macrophage, megakaryocyte and erythroid) with the additional appearance within 10days of large numbers of mature granulocytes, macrophages, and megakaryocytes, as well as occasional erythroid cells. Inclusion of flt3-ligand, Steelfactor, IL-3, IL-6, and G-CSF +/- erythropoietin (EPO) in the cultures enhanced only slightly the output of mature cells (except for the erythroid population which was much larger when EPO was added), Analogous subpopulations of normal CD34(+) cells produced similar numbers and types of cells but, as expected, only when growth factors were added. Thus primitive CD34(+) CML cells proliferating autonomously in vitro recapitulate the full spectrum of differentiation responses of normal CD34(+) cells stimulated by IL-3 andG-CSF, These findings point to a role of autocrine IL-3 and G-CSF in the similar multi-lineage expansion of differentiating CD34(+) CML cells that occurs in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 14:32:47