Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Diagnosis of human caliciviruses by use of enzyme immunoassays
Autore:
Jiang, X; Wilton, N; Zhong, WM; Farkas, T; Huang, PW; Barrett, E; Guerrero, M; Ruiz-Palacios, G; Green, KY; Green, J; Hale, AD; Estes, MK; Pickering, LK; Matson, DO;
Indirizzi:
Eastern Virginia Med Sch, Childrens Hosp Kings Daughters, Ctr Pediat Res, Norfolk, VA 23510 USA Eastern Virginia Med Sch Norfolk VA USA 23510 Res, Norfolk, VA 23510 USA Dept Hlth, Richmond, VA USA Dept Hlth Richmond VA USADept Hlth, Richmond, VA USA Inst Nutr, Dept Infect Dis, Mexico City, DF, Mexico Inst Nutr Mexico CityDF Mexico Dept Infect Dis, Mexico City, DF, Mexico NIAID, Infect Dis Lab, NIH, Bethesda, MD 20892 USA NIAID Bethesda MD USA 20892 , Infect Dis Lab, NIH, Bethesda, MD 20892 USA Baylor Coll Med, Div Virol, Houston, TX USA Baylor Coll Med Houston TX USA ylor Coll Med, Div Virol, Houston, TX USA Cent Publ Hlth Lab, Virus Reference Lab, London NW9 5HT, England Cent PublHlth Lab London England NW9 5HT e Lab, London NW9 5HT, England
Titolo Testata:
JOURNAL OF INFECTIOUS DISEASES
, volume: 181, anno: 2000, supplemento:, 2
pagine: S349 - S359
SICI:
0022-1899(200005)181:<S349:DOHCBU>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
ROUND-STRUCTURED VIRUSES; NORWALK-LIKE VIRUS; IMMUNE ELECTRON-MICROSCOPY; SNOW MOUNTAIN AGENT; LINKED IMMUNOSORBENT ASSAYS; CAPSID PROTEIN; MEXICO VIRUS; MOLECULAR CHARACTERIZATION; IMMUNOGLOBULIN-M; UNITED-KINGDOM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
83
Recensione:
Indirizzi per estratti:
Indirizzo: Jiang, X Eastern Virginia Med Sch, Childrens Hosp Kings Daughters, Ctr Pediat Res, 855 W Brambleton Ave, Norfolk, VA 23510 USA Eastern Virginia Med Sch 855 W Brambleton Ave Norfolk VA USA 23510
Citazione:
X. Jiang et al., "Diagnosis of human caliciviruses by use of enzyme immunoassays", J INFEC DIS, 181, 2000, pp. S349-S359

Abstract

The application of molecular technologies, such as the expression of viralproteins in baculovirus, has provided a powerful approach to the diagnosisof human calicivirus (HuCV) infections, The baculovirus-expressed HuCV capsid protein self-assembles into virus-like particles, providing excellent reagents for immunologic assays, such as enzyme immunoassays (EIAs), Following the expression of the capsid protein of Norwalk virus, the capsid proteins of 8 other HuCV strains have been expressed in baculovirus. The unlimited supply of baculovirus-produced reagents for HuCVs allows these EIAs to beapplied in large-scale clinical and epidemiological studies. Both the antigen and antibody-detection EIAs are highly sensitive. The antigen-detectionEIAs are highly specific, but the antibody-detection EIAs are more broadlyreactive. This article reviews baculovirus expression techniques used to produce HuCV capsid antigens, development of EIAs using these antigens, and application of these EIAs in studies of HuCV infection and illness.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 16:58:49