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Titolo:
Sensitive liquid chromatographic assay for amprenavir, a human immunodeficiency virus protease inhibitor, in human plasma, cerebrospinal fluid and semen
Autore:
Sparidans, RW; Hoetelmans, RMW; Beijnen, JH;
Indirizzi:
Univ Utrecht, Fac Pharm, Div Drug Toxicol, NL-3584 CA Utrecht, NetherlandsUniv Utrecht Utrecht Netherlands NL-3584 CA 3584 CA Utrecht, Netherlands Slotervaart Hosp, Netherlands Canc Inst, Dept Pharm & Pharmacol, NL-1066 EC Amsterdam, Netherlands Slotervaart Hosp Amsterdam Netherlands NL-1066 EC Amsterdam, Netherlands
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 742, anno: 2000,
pagine: 185 - 192
SICI:
1387-2273(20000526)742:1<185:SLCAFA>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOLID-PHASE EXTRACTION; ULTRAVIOLET DETECTION; INDINAVIR; SAQUINAVIR; RITONAVIR; NELFINAVIR; 141W94;
Keywords:
amprenavir; protease inhibitor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Sparidans, RW Univ Utrecht, Fac Pharm, Div Drug Toxicol, Sorbonnelaan 16, NL-3584 CA Utrecht, Netherlands Univ Utrecht Sorbonnelaan 16 Utrecht Netherlands NL-3584 CA
Citazione:
R.W. Sparidans et al., "Sensitive liquid chromatographic assay for amprenavir, a human immunodeficiency virus protease inhibitor, in human plasma, cerebrospinal fluid and semen", J CHROMAT B, 742(1), 2000, pp. 185-192

Abstract

A sensitive bio-analytical assay for amprenavir, a human immunodeficiency virus protease inhibitor, based on reversed-phase liquid chromatography andfluorescence detection, is reported. The analyte is extracted from the matrix, plasma, cerebrospinal fluid (CSF) or semen, with chloroform using propyl-p-hydroxybenzoate as an internal standard. After centrifugation, evaporation of the organic phase and reconstitution in the eluent, the sample is injected into the chromatograph. The analyte is detected spectrofluorometrically at 270 and 340 nm for excitation and emission, respectively. The method has been validated in the 1-1000 ng/ml range for a 50-mu l volume of plasma and in the 0.5-50 ng/ml range for a 100-mu l volume of CSF and semen. The lower limit of quantification was 0.5 ng/ml in CSF and 1 ng/ml in both plasma and semen. Precision and accuracy both meet the current requirements for a bio-analytical assay and are (15% in the validated ranges. The assay was successfully used to obtain a concentration-time curve of amprenavir in plasma. (C) 2000 Elsevier Science BN. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 00:39:56