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Titolo:
Transient induction of ENC-1, a Kelch-related actin-binding protein, is required for adipocyte differentiation
Autore:
Zhao, L; Gregoire, F; Sul, HS;
Indirizzi:
Univ Calif Berkeley, Dept Nutr Sci, Berkeley, CA 94720 USA Univ Calif Berkeley Berkeley CA USA 94720 utr Sci, Berkeley, CA 94720 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 22, volume: 275, anno: 2000,
pagine: 16845 - 16850
SICI:
0021-9258(20000602)275:22<16845:TIOEAK>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-EXPRESSION; ADIPOSE CONVERSION; TRIGLYCERIDE ACCUMULATION; 3T3-L1 PREADIPOCYTES; INTERACTION MOTIF; MESSENGER-RNA; CELL-SHAPE; DOMAIN; FIBRONECTIN; COLLAGEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Sul, HS Univ Calif Berkeley, Dept Nutr Sci, Berkeley, CA 94720 USA Univ Calif Berkeley Berkeley CA USA 94720 Berkeley, CA 94720 USA
Citazione:
L. Zhao et al., "Transient induction of ENC-1, a Kelch-related actin-binding protein, is required for adipocyte differentiation", J BIOL CHEM, 275(22), 2000, pp. 16845-16850

Abstract

In an attempt to study molecules that play a regulatory role early in adipocyte differentiation, we identified by differential display ENC-1, a Drosophila kelch-related protein. ENC-1 colocalizes with actin filaments. ENC-1 is expressed in adipose tissue, specifically in the adipose derived stroma vascular fraction. ENC-1 mRNA levels are transiently increased 8-12-fold early in in vitro adipocyte differentiation of primary cells of the adipose-derived stroma-vascular fraction and of 3T3-L1 cells. Treatment with the adipogenic inducers dexamethasone and methylisobutylxanthine causes an increase in ENC-1 mRNA levels specifically in preadipocytes, and methylisobutylxanthine is the main effector of ENC-1 expression. The induction of ENC-1 precedes expression of the transcription factors, peroxisome proliferator-activated receptor (PPAR gamma) and CCAAT/enhancer-binding protein (C/EBP alpha), and other adipocyte markers including adipocyte fatty acid-binding protein. The ENC-1 induction correlates with the subsequent differentiation of primary stroma-vascular cells into adipocytes. Furthermore, decreasing the endogenous ENC-1 levels by stable antisense transfection, thereby preventing the transient induction, effectively inhibits 3T3-L1 adipocyte differentiation. Overall, these studies indicate that ENC-1, an actin-binding protein, plays a regulatory role early in adipocyte differentiation when cytoskeletal reorganization and cell shape change from fibroblastic preadipocytes to spherical adipocytes occur.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 03:31:07