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Titolo:
Molecular cloning and characterization of SRAM, a novel insect Rel/Ankyrin-family protein present in nuclei
Autore:
Shiraishi, H; Kobayashi, A; Sakamoto, Y; Nonaka, T; Mitsui, Y; Aozasa, N; Kubo, T; Natori, S;
Indirizzi:
Inst Phys & Chem Res, Wako, Saitama 3510198, Japan Inst Phys & Chem Res Wako Saitama Japan 3510198 o, Saitama 3510198, Japan Univ Tokyo, Grad Sch Pharmaceut Sci, Dept Cell Biochem, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 Biochem, Bunkyo Ku, Tokyo 1130033, Japan Osaka Univ, Grad Sch Pharmaceut Sci, Biochem & Mol Biol Lab, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 iol Lab, Suita, Osaka 5650871, Japan Nagaoka Univ Technol, Dept Bioengn, Nagaoka, Niigata 9402137, Japan Nagaoka Univ Technol Nagaoka Niigata Japan 9402137 Niigata 9402137, Japan
Titolo Testata:
JOURNAL OF BIOCHEMISTRY
fascicolo: 6, volume: 127, anno: 2000,
pagine: 1127 - 1134
SICI:
0021-924X(200006)127:6<1127:MCACOS>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
NF-KAPPA-B; SARCOPHAGA LECTIN GENE; EMBRYONIC-CELL LINE; PEREGRINA FLESH FLY; DNA-BINDING; ANTIBACTERIAL PROTEINS; TRANSCRIPTION FACTORS; DROSOPHILA IMMUNITY; ANOPHELES-GAMBIAE; INNATE IMMUNITY;
Keywords:
ankyrin; insect immunity; kappa B motif; NF-kappa B/Rel family; Sarcophaga peregrina;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Natori, S Inst Phys & Chem Res, Wako, Saitama 3510198, Japan Inst Phys & Chem Res Wako Saitama Japan 3510198 3510198, Japan
Citazione:
H. Shiraishi et al., "Molecular cloning and characterization of SRAM, a novel insect Rel/Ankyrin-family protein present in nuclei", J BIOCHEM, 127(6), 2000, pp. 1127-1134

Abstract

Previously, we purified a 59-kDa protein that binds to the KB motif of theSarcophaga lectin gene. Here we report its cDNA cloning and some of its characteristics as a novel member of the Rel/Ankyrin-family. This protein, named SRAM, contained a Rel homology domain, a nuclear localization signal and 4 ankyrin repeats, but lacked the Ser-rich domain and PEST sequence that Relish contained, We found that SRAM was localized in the nuclei of NIH-Sape-4 cells, which are an embryonic cell line of Sarcophaga, The Sarcophaga lectin gene promoter containing tandem repeats of the kappa B moths was activated in NIH-Sape-4 cells. In Drosophila mbn-2 cells, Dif alone activated this reporter gene and a cooperative effect was detected when SRAM and Dif were co-transfected, although SRAM alone did not activate it. This is the first report of a Rel/Ankyrin molecule that exists in the nuclei.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 05:02:04