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Titolo:
FORMATION OF THE EARLY-REGION-2 TRANSCRIPTION-FACTOR-1-RETINOBLASTOMA-PROTEIN (E2F-1-RB) TRANSREPRESSOR AND RELEASE OF THE RETINOBLASTOMA PROTEIN FROM NUCLEAR-COMPLEXES CONTAINING CYCLIN A IS INDUCED BY INTERFERON-ALPHA IN U937V CELLS BUT NOT IN INTERFERON-ALPHA-RESISTANT U937VRCELLS
Autore:
KIRCH HC; PUTZER B; BROCKMANN D; ESCHE H; KLOKE O;
Indirizzi:
UNIV ESSEN GESAMTHSCH,SCH MED,W GERMAN CANC CTR,INST MOL BIOL,DEPT MOL BIOL,HUFELANDSTR 55 D-45122 ESSEN GERMANY UNIV ESSEN GESAMTHSCH,SCH MED,W GERMAN CANC CTR,DEPT INTERNAL MED D-45122 ESSEN GERMANY
Titolo Testata:
European journal of biochemistry
fascicolo: 3, volume: 246, anno: 1997,
pagine: 736 - 744
SICI:
0014-2956(1997)246:3<736:FOTET>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
SENSITIVE HEMATOPOIETIC-CELLS; CHRONIC MYELOGENOUS LEUKEMIA; DNA-BINDING ACTIVITY; TRANSCRIPTION FACTOR; KINASE-C; RB PROTEIN; TYROSINE PHOSPHORYLATION; INTERLEUKIN-6 SUPPRESS; DAUDI CELLS; E2F;
Keywords:
INTERFERON; CELL CYCLE; RETINOBLASTOMA PROTEIN; EARLY-REGION-2 TRANSCRIPTION FACTOR; CYCLIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
H.C. Kirch et al., "FORMATION OF THE EARLY-REGION-2 TRANSCRIPTION-FACTOR-1-RETINOBLASTOMA-PROTEIN (E2F-1-RB) TRANSREPRESSOR AND RELEASE OF THE RETINOBLASTOMA PROTEIN FROM NUCLEAR-COMPLEXES CONTAINING CYCLIN A IS INDUCED BY INTERFERON-ALPHA IN U937V CELLS BUT NOT IN INTERFERON-ALPHA-RESISTANT U937VRCELLS", European journal of biochemistry, 246(3), 1997, pp. 736-744

Abstract

We have analysed the different regulation of cell-cycle-relevant proteins by interferon alpha (IFN alpha) in IFN alpha-sensitive and resistant U937 leukemic cell lines. In contrast to the IFN alpha-sensitive U937 variant cell line U937V, the IFN alpha-resistant derivative (U937VR) is insensitive to the antiproliferative activity of IFN alpha. As we found no differences between these cell lines concerning the induction by IFN alpha of the pathway involving tyrosine-protein kinases and the signal transducer and activator of transcription (Jak-Stat): we examined whether cell-cycle-regulating proteins are differently affectedby IFN alpha in U937V and U937VR cells, In U937V cells IFN alpha induced the formation of the complex between early-region-2 transcription factor 1 (E2F-1) and retinoblastoma protein (RB) which is known to repress transcription of E2F-1-inducible genes, necessary for cell cycle progression, Formation of this complex was not inducible by IFN alpha in U937VR cells, although the suitable binding partners (E2F-1 and under-phosphorylated RE) were present. Interestingly. treatment of nuclear extracts from logarithmically growing U937V and U937VR cells with anantiserum against cyclin A that disrupts cyclin-A-containing complexes, led to the formation of the E2F-1-RB complex, suggesting the presence of under-phosphorylated (active) RB, trapped in nuclear complexes that contain cyclin A, This suggestion was supported by combined immunoprecipitation/western blot experiments that revealed a physical interaction between phosphorylated as well as under-phosphorylated forms of RB and cyclin A complex(es) in U937V and U937VR cells. RE, especially the under-phosphorylated form, was released by treatment with IFN alpha from this complex(es) in the case of U937V cells but not U937VR cells. We conclude that the missing induction of the E2F-1-RB transrepressor by IFN alpha and the failure to release RB from cyclin-A-containingcomplexes might contribute to the resistance of U937VR cells to the antiproliferative effects of IFN alpha.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 11:46:49