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Titolo:
Reduction of intramembranous particles in the periacrosomal plasma membrane of boar spermatozoa during in vitro capacitation: A statistical study
Autore:
Suzuki-Toyota, F; Itoh, Y; Naito, K;
Indirizzi:
Chiba Univ, Sch Med, Dept Anat & Dev Biol, Chiba 2608670, Japan Chiba Univ Chiba Japan 2608670 ept Anat & Dev Biol, Chiba 2608670, Japan Tokyo Metropolitan Livestock Expt Stn, Tokyo 1980024, Japan Tokyo Metropolitan Livestock Expt Stn Tokyo Japan 1980024 1980024, Japan Univ Tokyo, Grad Sch Agr & Life Sci, Dept Anim Resource Sci, Lab Appl Genet, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 Sci, Lab Appl Genet, Tokyo 1138657, Japan
Titolo Testata:
DEVELOPMENT GROWTH & DIFFERENTIATION
fascicolo: 3, volume: 42, anno: 2000,
pagine: 265 - 273
SICI:
0012-1592(200006)42:3<265:ROIPIT>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
GUINEA-PIG SPERMATOZOA; INVITRO CAPACITATION; ACROSOME REACTION; HUMAN-SPERM; FERTILIZATION; CHOLESTEROL; MATURATION; STEROLS; SURFACE; FUSION;
Keywords:
boar spermatozoa; capacitation; cholesterol; freeze-fracture; membrane remodeling;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Suzuki-Toyota, F Chiba Univ, Sch Med, Dept Anat & Dev Biol, Chiba 2608670,Japan Chiba Univ Chiba Japan 2608670 ol, Chiba 2608670, Japan
Citazione:
F. Suzuki-Toyota et al., "Reduction of intramembranous particles in the periacrosomal plasma membrane of boar spermatozoa during in vitro capacitation: A statistical study", DEVELOP GR, 42(3), 2000, pp. 265-273

Abstract

Membrane remodeling in the periacrosomal plasma membrane (PAPM) of boar spermatozoa during incubation in capacitation medium was examined by the freeze-fracture technique. In the preservation medium (PM) group, the major small (about 8 nm) intramembranous particles (IMP) and the minor large (> 10 nm) IMP were distributed evenly in the PAPM. The IMP-free area increased during capacitation. To correct the IMP-free area, arithmetically redistributed (ARD)-IMP density was used for statistical analysis. In the PM group, themean density +/- SD of large IMP was 379 +/- 64 and 266 +/- 58/mu m(2), and that of small IMP was 1450 +/- 155 and 672 +/- 252/mu m(2) in protoplasmic (P) and external (E) faces, respectively. During capacitation, the significant (P < 0.01) reduction of large IMP density was encountered only in theE face of a few incubation groups, while that of the small IMP density occurred in the P face by 2 h. Consequently, reduction of the total IMP density of both faces was not significant in the large IMP, but it was significant (P < 0.01) in the small IMP. One-fifth of the total small IMP density reduced by 2 h. Filipin-sterol complexes (FSC) were numerous in the PAPM, and FSC-free areas also increased during capacitation. The mechanism of IMP-free area formation and the behavior of the small IMP in the PAPM during capacitation were discussed in relation to membrane stability.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:03:17