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Titolo:
Apolipoprotein E gene expression correlates with endogenous lipid nutrition and yolk syncytial layer lipoprotein synthesis during fish development
Autore:
Poupard, G; Andre, M; Durliat, M; Ballagny, C; Boeuf, G; Babin, PJ;
Indirizzi:
Univ Paris Sud, CNRS, UPRESA 8080, F-91405 Orsay, France Univ Paris Sud Orsay France F-91405 , UPRESA 8080, F-91405 Orsay, France IFREMER, Ctr Brest, Stn Ressources Vivantes, F-29280 Plouzane, France IFREMER Plouzane France F-29280 urces Vivantes, F-29280 Plouzane, France
Titolo Testata:
CELL AND TISSUE RESEARCH
fascicolo: 2, volume: 300, anno: 2000,
pagine: 251 - 261
SICI:
0302-766X(200005)300:2<251:AEGECW>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
SCOPHTHALMUS-MAXIMUS L; RECEPTOR-BINDING DOMAIN; FATTY-ACID COMPOSITION; STRIPED BASS LARVAE; E MESSENGER-RNA; A-I GENES; EMBRYONIC-DEVELOPMENT; SALMO-GAIRDNERI; ZEBRAFISH BLASTOMERES; ENERGY-METABOLISM;
Keywords:
ApoE; larva; oil globule; yolk; lipoprotein; zebrafish; turbot, Scophthalmus maximus (Teleostei);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
87
Recensione:
Indirizzi per estratti:
Indirizzo: Babin, PJ Univ Paris Sud, CNRS, UPRESA 8080, Bat 447, F-91405 Orsay, France Univ Paris Sud Bat 447 Orsay France F-91405 91405 Orsay, France
Citazione:
G. Poupard et al., "Apolipoprotein E gene expression correlates with endogenous lipid nutrition and yolk syncytial layer lipoprotein synthesis during fish development", CELL TIS RE, 300(2), 2000, pp. 251-261

Abstract

During embryogenesis of teleost fish, the formation of a yolk syncytial layer (YSL) enables the resorption of the yolk reserves and development up tothe larval stage. We have examined the changes of the yolk cell structure in relation to yolk and oil-globule lipid utilization during development ofthe turbot (Scophthalmus maximus). After encapsulation by the YSL, resorption of the single, large oil globule occurred predominantly after yolk resorption and was slower in fasting larvae. The YSL was in contact with an enlarged perisyncytial space, but no vascular network or red blood cells were present within the walls of the yolk sac. Intrasyncytial channels infiltrated by pigmented lining cells were observed in the YSL surrounding the oil globule. Apolipoprotein E (apoE) has a prominent role in lipid metabolism because of its ability to interact with lipoprotein receptors. We performed molecular cloning of the putative low-density lipoprotein-receptor binding domain of turbot apoE. In situ hybridization analysis revealed a very high level of apoE transcripts in the YSL, while no expression could be detected in the intestine. YSL apoE expression was correlated with the synthesis of very low density Lipoprotein (VLDL) particles. An extraordinarily high number of VLDL particles were poured into the perisyncytial space, and intrasyncytial channels enabled the transfer of yolk- and oil globule-derived lipids to the developing embryo or larva. The pattern of apoE mRNA distribution in relation to YSL lipoprotein synthesis indicates that apoE expression is a suitable molecular marker for monitoring endogenous lipid nutrition during the endo-exotrophic period of teleost fish development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 12:21:29