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Titolo:
The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage
Autore:
Kina, T; Ikuta, K; Takayama, E; Wada, K; Majumdar, AS; Weissman, IL; Katsura, Y;
Indirizzi:
Kyoto Univ, Inst Frontier Med Sci, Dept Immunol, Sakyo Ku, Kyoto 6068507, Japan Kyoto Univ Kyoto Japan 6068507 t Immunol, Sakyo Ku, Kyoto 6068507, Japan Kyoto Univ, Sch Med, Dept Med Chem, Kyoto 6068507, Japan Kyoto Univ Kyoto Japan 6068507 Med, Dept Med Chem, Kyoto 6068507, Japan Stanford Univ, Sch Med, Dept Pathol, Stanford, CA 94305 USA Stanford UnivStanford CA USA 94305 , Dept Pathol, Stanford, CA 94305 USA
Titolo Testata:
BRITISH JOURNAL OF HAEMATOLOGY
fascicolo: 2, volume: 109, anno: 2000,
pagine: 280 - 287
SICI:
0007-1048(200005)109:2<280:TMATRA>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC STEM-CELLS; ERYTHROLEUKEMIA-CELLS; MOUSE GLYCOPHORIN; CDNA CLONES; DIFFERENTIATION; EXPRESSION; IDENTIFICATION; MEMBRANE; SEQUENCE;
Keywords:
haematopoietic cells; lineage marker; erythroid cell; monoclonal antibody glycophorin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Kina, T Kyoto Univ, Inst Frontier Med Sci, Dept Immunol, Sakyo Ku, Kyoto 6068507, Japan Kyoto Univ Kyoto Japan 6068507 l, Sakyo Ku, Kyoto 6068507, Japan
Citazione:
T. Kina et al., "The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage", BR J HAEM, 109(2), 2000, pp. 280-287

Abstract

The antigen specificity of a rat monoclonal antibody TER-119 was investigated. In adult mice, TER-119 reacted with mature erythrocytes, 20-25% of bone marrow cells and 2-3% of spleen cells but not with thymocytes nor lymph node cells. In fetal haematopoietic tissues, 30-40% of d 10 yolk sac cells, 80-90% of d 14 fetal liver cells and 40-50% of newborn liver cells were reactive with TER-119. TER-119(+) cells in adult bone marrow ex-pressed significant levels of CD45 but not myeloid (Mac-1, Gr-1) or B-cell (B220) markers. Morphological examination and haematopoietic colony-forming assays for isolated TER-119(+) cells revealed that TER-119 reacts with erythroid cells at differentiation stages from early proerythroblast to mature erythrocyte, but not with cells showing typical erythroid blast-forming unit (BFU-E) anderythroid colony-forming unit (CFU-E) activities. Erythroleukaemia cell lines do not express the TER-119 antigen even after stimulation with dimethylsulphoxide. TER-119 immunoprecipitated protein bands with molecular masses of 110 kDa, 60 kDa, 52 kDa and 32 kDa from erythrocyte membrane, whereas only a 52-kDa band was detected by TER-119 in Western blot analysis. Further molecular and cellular analyses indicated that the TER-119 antigen is a molecule associated with cell-surface glycophorin A but not with glycophorin Aitself.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/01/20 alle ore 12:44:25