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Titolo:
Comparative fatty acid selectivity of lipases in esterification reactions with glycerol and diol analogues in organic media
Autore:
Lee, CH; Parkin, KL;
Indirizzi:
Univ Wisconsin, Dept Food Sci, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 Dept Food Sci, Madison, WI 53706 USA
Titolo Testata:
BIOTECHNOLOGY PROGRESS
fascicolo: 3, volume: 16, anno: 2000,
pagine: 372 - 377
SICI:
8756-7938(200005/06)16:3<372:CFASOL>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
PSEUDOMONAS-CEPACIA LIPASE; X-RAY CRYSTALLOGRAPHY; CANDIDA-RUGOSA; MICROBIAL LIPASES; SUBSTRATE SELECTIVITY; ENZYMATIC CATALYSIS; HUMICOLA-LANUGINOSA; STRUCTURAL BASIS; ESTER SYNTHESIS; SPECIFICITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Parkin, KL Univ Wisconsin, Dept Food Sci, 1605 Linden Dr, Madison, WI 53706 USA Univ Wisconsin 1605 Linden Dr Madison WI USA 53706 WI 53706 USA
Citazione:
C.H. Lee e K.L. Parkin, "Comparative fatty acid selectivity of lipases in esterification reactions with glycerol and diol analogues in organic media", BIOTECH PR, 16(3), 2000, pp. 372-377

Abstract

Reaction selectivity of Pseudomonas cepacia, Rhizomucor miehei, and Candida antarctica B lipases was assessed in multicompetitive esterification reaction mixtures containing an homologous series of 12-chain even carbon number fatty acid (FA; C4-C18) substrates and a single alcohol cosubstrate (glycerol, 1,2-propanediol (1,2-PD), or 1,3-propanediol (1,3-PD)) in tert-butyl methyl ether at water activity of 0.69 or 0.90 and a reaction temperature of 35 degrees C. For P. cepacia lipase, the ordinal patterns of FA selectivities observed were, with glycerol, C8 > C10, C6, C16 > other FA; with 1,2-PD and 1,3-PD, C16 > C8 > C14 > other FA. For R. miehei lipase, the ordinal patterns of FA selectivities observed were, with glycerol, C8 > C12 > C10, C14 > other FA; with 1,2-PD and 1,3-PD, C8 > C12 > other FA. For C. antarctica B lipase, the ordinal patterns of FA selectivities observed were, with glycerol, C8 > C10, C6, C12 > other FA; with 1,2-PD, C8 > C10, C6 > other FA; and with 1,3-PD, C8 > C10 > C6 > other FA. The differences in selectivity among FA ranged up to le-fold, depending upon the lipase and alcohol cosubstrate used. These findings represent intrinsic and substrate-modulated features of FA selectivities that are of particular relevance to the use of lipases for acylglycerol synthesis reactions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 20:04:55