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Titolo:
The growth factor inhibitor suramin reduces apoptosis and cell aggregationin protein-free CHO cell batch cultures
Autore:
Zanghi, JA; Renner, WA; Bailey, JE; Fussenegger, M;
Indirizzi:
ETH Honggerberg, Inst Biotechnol, Swiss Fed Inst Technol, HPT, CH-8093 Zurich, Switzerland ETH Honggerberg Zurich Switzerland CH-8093 , CH-8093 Zurich, Switzerland
Titolo Testata:
BIOTECHNOLOGY PROGRESS
fascicolo: 3, volume: 16, anno: 2000,
pagine: 319 - 325
SICI:
8756-7938(200005/06)16:3<319:TGFISR>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHINESE-HAMSTER OVARY; ALKALINE-PHOSPHATASE; SURFACE RECEPTOR; GENE-EXPRESSION; MAMMALIAN-CELLS; BINDING; SERUM; PROLIFERATION; DEPRIVATION; SUSPENSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Zanghi, JA ETH Honggerberg, Inst Biotechnol, Swiss Fed Inst Technol, HPT, CH-8093 Zurich, Switzerland ETH Honggerberg Zurich Switzerland CH-8093 urich, Switzerland
Citazione:
J.A. Zanghi et al., "The growth factor inhibitor suramin reduces apoptosis and cell aggregationin protein-free CHO cell batch cultures", BIOTECH PR, 16(3), 2000, pp. 319-325

Abstract

We have previously shown that Chinese hamster ovary (CHO) cells capable ofgrowing in medium free of exogenous proteins die by apoptosis during all stages of a batch culture (Zanghi et al., 1999). On the basis of the hypothesis that extracellular death factors might be important in apoptosis under these conditions, we examined the effect of the growth factor inhibitor andantitumor agent suramin on CHO cell growth and apoptosis in serum-free culture. Suramin protected against apoptosis during exponential growth, as indicated by the absence of DNA laddering and an increase in cell viability from roughly 70% to above 95%. Suramin also effectively dispersed cell aggregates so that single-cell suspension culture was possible. However, suramin did not protect; against apoptosis during the death phase, in contrast to serum, suggesting that antiapoptotic factors in the serum remain to be discovered. The increased viable cell yield following suramin supplementation resulted in a 40% increase in product yield, based on results with cells expressing recombinant secreted alkaline phosphatase. Polysulfated compounds dextran sulfate and polyvinyl sulfate worked nearly as well as suramin in dispersing cell clumps and increasing viable cell yield, which implies that suramin's high sulfate group density may be responsible for its effects in cell culture. In addition, suramin was beneficial for long-term adaptation ofCHO cells to protein-free media suspension culture, and the compound was synergistic with insulin in accelerating this adaptation time.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 06:16:16