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Titolo:
Characterization of microorganisms and biomarker development from global ESI-MS/MS analyses of cell lysates
Autore:
Xiang, F; Anderson, GA; Veenstra, TD; Lipton, MS; Smith, RD;
Indirizzi:
Pacific NW Natl Lab, Environm Mol Sci Lab, Richland, WA 99352 USA Pacific NW Natl Lab Richland WA USA 99352 Sci Lab, Richland, WA 99352 USA
Titolo Testata:
ANALYTICAL CHEMISTRY
fascicolo: 11, volume: 72, anno: 2000,
pagine: 2475 - 2481
SICI:
0003-2700(20000601)72:11<2475:COMABD>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLIGHT MASS-SPECTROMETRY; ASSISTED-LASER-DESORPTION/IONIZATION; COMPLEX BIOLOGICAL SAMPLES; FAST-ATOM-BOMBARDMENT; RAPID IDENTIFICATION; ELECTROSPRAY IONIZATION; DUAL MICRODIALYSIS; WHOLE CELLS; BACTERIA; PROTEINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Smith, RD Pacific NW Natl Lab, Environm Mol Sci Lab, POB 999, Richland, WA99352 USA Pacific NW Natl Lab POB 999 Richland WA USA 99352 , WA 99352 USA
Citazione:
F. Xiang et al., "Characterization of microorganisms and biomarker development from global ESI-MS/MS analyses of cell lysates", ANALYT CHEM, 72(11), 2000, pp. 2475-2481

Abstract

The capability for sensitive and accurate identification of microorganismshas potential applications that include the monitoring of industrial bioprocessing operations, food safety analyses, disease diagnosis, and detectionof potential biological hazards. Efforts based upon matrix-assisted laser desorption/ionization mass spectrometry to detect and identify specific microorganisms have been actively pursued for several years, We report a new method being developed to select useful biomarkers for the identification ofmicroorganisms based upon electrospray ionization (ESI)-ion trap mass spectrometry, Crude cell lysates are processed using a recently developed dual-microdialysis device and then directly infused into an ion trap MS, The lowESI flow rate and precursor ion accumulation capability of the ion trap MSenables high-sensitivity MS/MS analyses, Precursor ions are automatically selected and analyzed using tandem MS (MS/MS) to produce "global" MS/MS surveys and processed to yield two-dimensional MS/MS spectral displays. Such global MS/MS surveys are demonstrated for Escherichia coli lysates, The distinctive MS/MS spectral patterns can be used to identify mass spectrometric-detected species useful as biomarkers, which then provide a basis for confident microorganism identification. The results presented demonstrate the application of this method for the identification of microorganisms, as well as for detection of bacteriophage MS2 in the presence of a large excess of E. coli.

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Documento generato il 24/09/20 alle ore 04:39:07