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Titolo:
TOPOISOMERASE FUNCTION DURING REPLICATION-INDEPENDENT CHROMATIN ASSEMBLY IN YEAST
Autore:
GARINTHER WI; SCHULTZ MC;
Indirizzi:
UNIV ALBERTA,DEPT BIOCHEM EDMONTON AB T6G 2H7 CANADA UNIV ALBERTA,DEPT BIOCHEM EDMONTON AB T6G 2H7 CANADA
Titolo Testata:
Molecular and cellular biology
fascicolo: 7, volume: 17, anno: 1997,
pagine: 3520 - 3526
SICI:
0270-7306(1997)17:7<3520:TFDRCA>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA POLYMERASE-I; CELL-FREE SYSTEM; SACCHAROMYCES-CEREVISIAE; DNA TOPOISOMERASES; TRANSCRIPTION; PURIFICATION; CHROMOSOMES; INITIATION; EXTRACT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
W.I. Garinther e M.C. Schultz, "TOPOISOMERASE FUNCTION DURING REPLICATION-INDEPENDENT CHROMATIN ASSEMBLY IN YEAST", Molecular and cellular biology, 17(7), 1997, pp. 3520-3526

Abstract

DNA topoisomerases I and II are the two major nuclear enzymes capableof relieving torsional strain in DNA, Of these enzymes, topoisomeraseI plays the dominant role in relieving torsional strain during chromatin assembly in cell extracts from oocytes, eggs, and early embryos. We tested if the topoisomerases are used differentially during chromatin assembly in Saccharomyces cerevisiae by a combined biochemical and pharmacological approach. As measured by plasmid supercoiling, nucleosome deposition is severely impaired in assembly extracts from a yeast mutant with no topoisomerase I and a temperature-sensitive form of topoisomerase II (strain top1-top2), Expression of wild-type topoisomeraseII in strain top1-top2 fully restored assembly-driven supercoiling, and assembly was equally efficient in extracts from strains expressing either topoisomerase I or II alone, Supercoiling in top1-top2 extract was rescued by adding back either purified topoisomerase I or II, Using the topoisomerase II poison VP-16, we show that topoisomerase II activity during chromatin assembly is the same in the presence and absence of topoisomerase I, We conclude that both topoisomerases I and II can provide the DNA relaxation activity required for efficient chromatinassembly in mitotically cycling yeast cells.

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Documento generato il 25/11/20 alle ore 07:17:05