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Titolo:
Murine stromal cell line HESS-5 maintains reconstituting ability of ex vivo-generated hematopoietic stem cells from human bone marrow and cytokine-mobilized peripheral blood
Autore:
Shimakura, Y; Kawada, H; Ando, K; Sato, T; Nakamura, Y; Tsuji, T; Kato, S; Hotta, T;
Indirizzi:
Tokai Univ, Sch Med, Dept Internal Med, Div Hematol, Kanagawa 2591193, Japan Tokai Univ Kanagawa Japan 2591193 , Div Hematol, Kanagawa 2591193, Japan Tokai Univ, Sch Med, Res Ctr Genet Engn & Cell Transplantat, Kanagawa 2591193, Japan Tokai Univ Kanagawa Japan 2591193 Transplantat, Kanagawa 2591193, Japan Tokai Univ, Sch Med, Dept Pediat, Kanagawa 2591193, Japan Tokai Univ Kanagawa Japan 2591193 , Dept Pediat, Kanagawa 2591193, Japan
Titolo Testata:
STEM CELLS
fascicolo: 3, volume: 18, anno: 2000,
pagine: 183 - 189
SICI:
1066-5099(2000)18:3<183:MSCLHM>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM-CULTURE; COMBINED IMMUNODEFICIENT MICE; PROGENITOR CELLS; CORD-BLOOD; REPOPULATING CELLS; INITIATING CELLS; IN-VITRO; EXPANSION; INTERLEUKIN-3; MAINTENANCE;
Keywords:
stem cell; peripheral blood; bone marrow; HESS-5; stromal cell; SRC;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Ando, K Tokai Univ, Sch Med, Dept Internal Med, Div Hematol, Kanagawa 2591193, Japan Tokai Univ Kanagawa Japan 2591193 matol, Kanagawa 2591193, Japan
Citazione:
Y. Shimakura et al., "Murine stromal cell line HESS-5 maintains reconstituting ability of ex vivo-generated hematopoietic stem cells from human bone marrow and cytokine-mobilized peripheral blood", STEM CELLS, 18(3), 2000, pp. 183-189

Abstract

Human bone marrow (BM) or mobilized peripheral blood (mPB) CD34(+) cells have been shown to loose their stem cell quality during culture period more easily than those from cord blood (CB), We previously reported that human umbilical CB stem cells could effectively be expanded in the presence of human recombinant cytokines and a newly established murine bone marrow stromalcell line HESS-5. In this study we assessed the efficacy of this xenogeneic coculture system using human BM and mPB CD34(+) cells as materials. We measured the generation of CD34(+)CD38(-) cells and colony-forming units, andassessed severe-combined immunodeficient mouse-repopulating cell (SRC) activity using cells five days after serum-free cytokine-containing culture inthe presence or the absence of a direct contact with HESS-5 cells. As compared with the stroma-free culture, the xenogeneic coculture was significantly superior on expansion of CD34(+)CD38(-) cells and colony-forming cells and on maintenance of SRC activity. The PKH26 study demonstrated that cell division was promoted faster in cells cocultured with HESS-5 cells than in cells cultured without HESS-5 cells, These results indicate that HESS-5 supports rapid generation of primitive progenitor cells (PPC) and maintains reconstituting ability of newly generated stem cells during ex vivo culture irrespective of the source of samples. This xenogeneic coculture system will be useful for ex vivo manipulation such as gene transduction to promote cell division and the generation of PPC and to prevent loss of stem cell quality.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 11:23:58