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Titolo:
Gene transfer by particle bombardment to embryogenic cultures of Picea abies and the production of transgenic plantlets
Autore:
Clapham, D; Demel, P; Elfstrand, M; Koop, HU; Sabala, I; Von Arnold, S;
Indirizzi:
Swedish Univ Agr Sci, Dept Forest Genet, S-75007 Uppsala, Sweden Swedish Univ Agr Sci Uppsala Sweden S-75007 net, S-75007 Uppsala, Sweden Univ Munich, Inst Bot, D-80638 Munich, Germany Univ Munich Munich Germany D-80638 ch, Inst Bot, D-80638 Munich, Germany
Titolo Testata:
SCANDINAVIAN JOURNAL OF FOREST RESEARCH
fascicolo: 2, volume: 15, anno: 2000,
pagine: 151 - 160
SICI:
0282-7581(2000)15:2<151:GTBPBT>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
CINNAMYL ALCOHOL-DEHYDROGENASE; SOMATIC EMBRYOS; L KARST; AGROBACTERIUM-TUMEFACIENS; MEDIATED TRANSFORMATION; STABLE TRANSFORMATION; SPRUCE; EXPRESSION; PINUS; ACCELERATION;
Keywords:
Basta; Norway spruce; particle bombardment; Picea abies; transgenic plantlets;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Clapham, D Swedish Univ Agr Sci, Dept Forest Genet, Box 7027, S-75007 Uppsala, Sweden Swedish Univ Agr Sci Box 7027 Uppsala Sweden S-75007 a, Sweden
Citazione:
D. Clapham et al., "Gene transfer by particle bombardment to embryogenic cultures of Picea abies and the production of transgenic plantlets", SC J FOR R, 15(2), 2000, pp. 151-160

Abstract

A particle inflow gun enabled efficient production of transgenic plantletsof Picea abies from embryogenic suspension cultures. In transient assays, the Zea ubiquitin promoter was 12-16 times as active as the 35S promoter. For stable transformation, the plasmid pAHC25 contained the bar gene and thegusA gene, both driven by the Zea ubiquitin promoter. Cells were maintained from 1 to 3 h before bombardment on proliferation medium supplemented with 0.25 M myoinositol and, from day 8, supplemented with Basta as selective agent. Embryogenic colonies resistant to Basta appeared from two months after bombardment. Of over 100 independent Basta-resistant sublines tested, 65% expressed the co-transformed reporter gene, even when it was not linked to the selectable marker. Over 80% of the sublines retained their embryogenic potential. Of 11 transformants analyzed, 4 contained transgenes in low copy number (1-3), the rest contained transgenes in up to 15-20 copies. Over 200 Basta-resistant sublines from four cell lines have been established, ofwhich 138 are confirmed as transformed. Plantlets have been regenerated and grown on in pots.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 15:04:39