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Titolo:
Classification of fusiform neocortical interneurons based on unsupervised clustering
Autore:
Cauli, B; Porter, JT; Tsuzuki, K; Lambolez, B; Rossier, J; Quenet, B; Audinat, E;
Indirizzi:
Ecole Super Phys & Chim Ind, CNRS, UMR 7637, F-75231 Paris 05, France Ecole Super Phys & Chim Ind Paris France 05 37, F-75231 Paris 05, France Ecole Super Phys & Chim Ind, CNRS, UMR 9081, F-75231 Paris, France Ecole Super Phys & Chim Ind Paris France F-75231 , F-75231 Paris, France
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 11, volume: 97, anno: 2000,
pagine: 6144 - 6149
SICI:
0027-8424(20000523)97:11<6144:COFNIB>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT FRONTAL-CORTEX; CALCIUM-BINDING PROTEINS; SUBUNIT MESSENGER-RNAS; NONPYRAMIDAL CELLS; VISUAL-CORTEX; CEREBRAL-CORTEX; MORPHOLOGICAL-CHARACTERISTICS; KAINATE RECEPTORS; CA2+ PERMEABILITY; GABAERGIC NEURONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Rossier, J Ecole Super Phys & Chim Ind, CNRS, UMR 7637, 10 Rue Vauquelin, F-75231 Paris 05, France Ecole Super Phys & Chim Ind 10 Rue Vauquelin Paris France 05 e
Citazione:
B. Cauli et al., "Classification of fusiform neocortical interneurons based on unsupervised clustering", P NAS US, 97(11), 2000, pp. 6144-6149

Abstract

A classification of fusiform neocortical interneurons (n = 60) was performed with an unsupervised cluster analysis based on the comparison of multiple electrophysiological and molecular parameters studied by patch-clamp and single-cell multiplex reverse transcription-PCR in rat neocortical acute slices. The multiplex reverse transcription-PCR protocol was designed to detect simultaneously the expression of GAD65, GAD67, calbindin, parvalbumin, calretinin, neuropeptide Y, vasoactive intestinal peptide (VIP), somatostatin (SS), cholecystokinin, alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid, kainate, N-methyl-D-aspartate, and metabotropic glutamate receptor subtypes. Three groups of fusiform interneurons with distinctive features were disclosed by the cluster analysis. The first type of fusiform neuron (n= 12), termed regular spiking nonpyramidal (RSNP)-SS cluster, was characterized by a firing pattern of RSNP cells and by a high occurrence of SS. Thesecond type of fusiform neuron (n = 32), termed RSNP-VIP cluster, predominantly expressed VIP and also showed firing properties of RSNP neurons with accommodation profiles different from those of RSNP-SS cells. Finally, the last type of fusiform neuron (n = 16) contained a majority of irregular spiking-VIPergic neurons. In addition, the analysis of glutamate receptors revealed cell-type-specific expression profiles. This study shows that combinations of multiple independent criteria define distinct neocortical populations of interneurons potentially involved in specific functions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/05/20 alle ore 15:24:57