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Titolo:
Highly reduced protection against Streptococcus pneumoniae after deletion of a single heavy chain gene in mouse
Autore:
Mi, QS; Zhou, L; Schulze, DH; Fischer, RT; Lustig, A; Rezanka, LJ; Donovan, DM; Longo, DL; Kenny, JJ;
Indirizzi:
NIA, Immunol Lab, Cell Dev Sect B, NIH, Baltimore, MD 21224 USA NIA Baltimore MD USA 21224 Cell Dev Sect B, NIH, Baltimore, MD 21224 USA NIA, Transgen & Knockout Facil Sect, Gerontol Res Ctr, NIH, Baltimore, MD 21224 USA NIA Baltimore MD USA 21224 Gerontol Res Ctr, NIH, Baltimore, MD 21224 USA Univ Maryland, Sch Med, Dept Microbiol & Immunol, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 l & Immunol, Baltimore, MD 21201 USA Taishan Med Coll, Clin Immunol Lab, Taian 271000, Shandong, Peoples R China Taishan Med Coll Taian Shandong Peoples R China 271000 g, Peoples R China Taishan Med Coll, Dept Dermatol, Taian 271000, Shandong, Peoples R China Taishan Med Coll Taian Shandong Peoples R China 271000 g, Peoples R China
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 11, volume: 97, anno: 2000,
pagine: 6031 - 6036
SICI:
0027-8424(20000523)97:11<6031:HRPASP>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNE-DEFICIENT MICE; PHOSPHOCHOLINE-SPECIFIC ANTIBODIES; PNEUMOCOCCAL INFECTION; REPERTOIRE DIVERSITY; BACTERIAL-ANTIGENS; AGED MICE; PHOSPHORYLCHOLINE-ANTIBODY; MONOCLONAL-ANTIBODIES; SOMATIC MUTATION; SELF-BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Kenny, JJ NIA, Immunol Lab, Cell Dev Sect B, NIH, 5600 Nathan Shock Dr, Baltimore, MD 21224 USA NIA 5600 Nathan Shock Dr Baltimore MD USA 21224 re, MD 21224 USA
Citazione:
Q.S. Mi et al., "Highly reduced protection against Streptococcus pneumoniae after deletion of a single heavy chain gene in mouse", P NAS US, 97(11), 2000, pp. 6031-6036

Abstract

Phosphocholine (PC) is the immunodominant epitope found on the surface of Streptococcus pneumoniae (SPn). T15-idiotype Abs, whose heavy (H) chain variable region is encoded by the V1 gene, are dominant in the anti-PC response in adult mice and protect mice from lethal pneumococcal infection. The ability of anti-PC Abs using H chains other than the V1 H chain to protect against pneumococcal infection remains controversial. We generated V1(-/-) knockout mice to determine whether protective anti-PC Abs could be produced in the absence of the V1 gene. No anti-PC Abs were produced in V1(-/-) mire immunized with avirulent SPn; however, PC-BSA binding Abs were induced after immunization with PC-keyhole limpet hemocyanin but at significantly lowerlevels than those in wild-type mice. These Abs provided poor protection aga inst virulent SPn; th us, <25% of V1(-/-) mice survived challenge with 10(4) bacteria as compared with 100% survival of V1(+/+) mice. The anti-PC Abs in V1(-/-) mice were heteroclitic, binding to nitrophenyl-PC better than to PC. None of nine hybridomas produced from V1(-/-) mice provided passive protection. However, the V1(-/-) mice produced normal amounts of Ab to SPn proteins that can partially protect mice against SPn. These data indicate that the V1 gene is critical for the production of anti-PC Abs providing optimum protection against infection with SPn, and the V1(-/-) mice could be useful in unmasking epitopes other than the immunodominant PC epitope on SPncapable of providing cross protection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 15:25:13