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Titolo:
Cytoskeletal organization defects and abortive activation in human oocytesafter IVF and ICSI failure
Autore:
Rawe, VY; Olmedo, SB; Nodar, FN; Doncel, GD; Acosta, AA; Vitullo, AD;
Indirizzi:
IOSS, Ctr Estudios Ginecol & Reprod, RA-1055 Buenos Aires, DF, Argentina IOSS Buenos Aires DF Argentina RA-1055 A-1055 Buenos Aires, DF, Argentina Eastern Virginia Med Sch, Jones Inst Reprod Med, Dept Obstet & Gynecol, Norfolk, VA 23507 USA Eastern Virginia Med Sch Norfolk VA USA 23507 ecol, Norfolk, VA 23507 USA
Titolo Testata:
MOLECULAR HUMAN REPRODUCTION
fascicolo: 6, volume: 6, anno: 2000,
pagine: 510 - 516
SICI:
1360-9947(200006)6:6<510:CODAAA>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTRACYTOPLASMIC SPERM INJECTION; HUMAN INVITRO FERTILIZATION; UNFERTILIZED HUMAN OOCYTES; HUMAN MALE PRONUCLEUS; MOUSE OOCYTE; MICROTUBULE; CENTROSOME; CHROMATIN; CONFIGURATIONS; ABNORMALITIES;
Keywords:
abortive activation; cytoskeletal organization; fertilization failure; microtubules;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Rawe, VY IOSS, Ctr Estudios Ginecol & Reprod, RA-1055 Buenos Aires, DF, Argentina IOSS Buenos Aires DF Argentina RA-1055 enos Aires, DF, Argentina
Citazione:
V.Y. Rawe et al., "Cytoskeletal organization defects and abortive activation in human oocytesafter IVF and ICSI failure", MOL HUM REP, 6(6), 2000, pp. 510-516

Abstract

In this study, we analysed the distribution of beta tubulins to detect spindle and cytoplasmic microtubules, a acetylated tubulins for sperm microtubules and chromatin configuration in oocytes showing fertilization failure after conventional IVF or intracytoplasmic sperm injection (ICSI). A total of 450 human oocytes that failed to fertilize were studied 20-40 h after IVFor ICSI. In all, 287 oocytes were stained for immunofluorescence and chromosomal spreads were performed by Tarkowski's air-drying method in 163 IVF or ICSI oocytes that did not develop pronuclei after the extrusion of a second polar body. Immunofluorescence analysis showed that the main reason of fertilization failure after IVF was no sperm penetration (55.5%). The remaining oocytes showed different abnormal patterns, e.g. oocyte activation failure (15.1%) and defects in pronuclei apposition (19.2%). On the other hand,fertilization failure after ICSI was mainly associated to incomplete oocyte activation (39.9%), and to a lesser extent with defects in pronuclei apposition (22.6%) and failure of sperm penetration (13.3%). A further 13.3% ofthe ICSI oocytes arrested their development at the metaphase of the first mitotic division. The chromosomal spreads allowed the analysis of abortive activations, in which no pronuclei formed but a second polar body was extruded. Immunofluorescence and cytogenetic analysis provided a useful tool to improve infertility diagnosis and prognosis in each particular case.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 20:11:30