Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Presence of N-cadherin transcripts in mature spermatozoa
Autore:
Goodwin, LO; Karabinus, DS; Pergolizzi, RG;
Indirizzi:
N Shore Univ Hosp, Dept Res, NYU, Sch Med, Manhasset, NY 11030 USA N ShoreUniv Hosp Manhasset NY USA 11030 Sch Med, Manhasset, NY 11030 USA Univ Arizona, Dept Obstet & Gynecol, Tucson, AZ USA Univ Arizona Tucson AZ USA rizona, Dept Obstet & Gynecol, Tucson, AZ USA
Titolo Testata:
MOLECULAR HUMAN REPRODUCTION
fascicolo: 6, volume: 6, anno: 2000,
pagine: 487 - 497
SICI:
1360-9947(200006)6:6<487:PONTIM>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-ADHESION MOLECULES; ZONA-PELLUCIDA BINDING; HUMAN-SPERM; IN-VITRO; EMBRYONIC HISTOGENESIS; SIGNAL-TRANSDUCTION; ADHERENS JUNCTIONS; ACROSOME REACTION; CALCIUM-CHANNEL; MOUSE EMBRYOS;
Keywords:
adhesion; cadherins; RNA extraction from spermatozoa; sperm-oocyte fusion mechanisms;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
101
Recensione:
Indirizzi per estratti:
Indirizzo: Goodwin, LO N Shore Univ Hosp, Dept Res, NYU, Sch Med, Manhasset, NY 11030USA N Shore Univ Hosp Manhasset NY USA 11030 hasset, NY 11030 USA
Citazione:
L.O. Goodwin et al., "Presence of N-cadherin transcripts in mature spermatozoa", MOL HUM REP, 6(6), 2000, pp. 487-497

Abstract

The essential mechanism involved in sperm-oolemma fusion has yet to be elucidated. Recognition and binding is initiated by specific cell surface receptor engagement between gametes. Fusion between hamster oolemma and spermatozoa is prevented in the presence of trypsin in Ca2+-free media, as is oocyte activation, implicating a cadherin-like adhesion. Cadherins are a familyof Ca2+-dependent adhesion molecules that bind homotypically with their target, are morphoregulatory and function eptopically to affect tissue form and function. Cadherins and cadherin-associated molecules have been identified in testes and germinal cells, as well as ejaculated spermatozoa. Moreover, cadherins are also present in oocytes and may suggest a cadherin-mediated adhesion in sperm-oocyte interaction, We have detected antigenic epitopesrecognized by N-cadherin monoclonal antibodies diffusely distributed over the entire sperm head. In addition, Western blot analysis confirmed the presence of an antibody reactive peptide in spermatozoa, testis and ovary protein extracts at the expected molecular weight for authentic N-cadherin. Total RNA was isolated from mature motile spermatozoa, as well as ovary and testis tissue, and served as template for reverse transcription-polymerase chain reaction (RT-PCR) with N-cadherin specific primers. Alignment of sequences from PCR products of testis, ovary and spermatozoa with published N-cadherin sequence was identical except for occasional base changes. We intend to develop methods to analyse this transcript from smalt numbers of spermatozoa from a variety of donors to determine if defects in cadherin distribution or structure may predict reduced male fertility.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/10/20 alle ore 23:42:45