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Titolo:
Multiplex PCR detection of Campylobacter jejuni and Arcobacter butzleri infood products
Autore:
Winters, DK; Slavik, MF;
Indirizzi:
Univ Arkansas, Dept Poultry Sci, Fayetteville, AR 72701 USA Univ ArkansasFayetteville AR USA 72701 y Sci, Fayetteville, AR 72701 USA
Titolo Testata:
MOLECULAR AND CELLULAR PROBES
fascicolo: 2, volume: 14, anno: 2000,
pagine: 95 - 99
SICI:
0890-8508(200004)14:2<95:MPDOCJ>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHICKEN WASHES; FRESH PRODUCE; IDENTIFICATION; ENTERITIS; DISEASE; COLI;
Keywords:
polymerase chain reaction; C. jejuni; A. butzleri; multiplex PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Winters, DK Univ Arkansas, Dept Poultry Sci, Fayetteville, AR 72701 USA Univ Arkansas Fayetteville AR USA 72701 teville, AR 72701 USA
Citazione:
D.K. Winters e M.F. Slavik, "Multiplex PCR detection of Campylobacter jejuni and Arcobacter butzleri infood products", MOL CELL PR, 14(2), 2000, pp. 95-99

Abstract

Arcobacter is a recently described species, previously considered part of the Campylobacter family. A sensitive assay such as that provided by PCR could help to distinguish the closely related Arcobacter from Campylobacter. A PCR method to specifically detect both Campylobacter jejuni and Arcobacter butzleri in the same reaction tube has been developed. C. jejuni and A. butzleri were inoculated into a range of dairy products, raw and ready-to-eat foods. The presence of these two organisms was detected in these test foods by the multiplex PCR assay. A product of 159 bp was apparent when C. jejuni was present, while a 1223 bp product was seen when A. butzleri was present. When both organisms were present, both bands could be detected on the agarose gel. All organisms were confirmed by standard microbiological methods. There was complete agreement between the PCR and standard methods. ThisPCR assay will allow detection of both organisms within the same PCR tube and can be performed within an 8 h day. The presence of these two human pathogens, which are difficult to distinguish by standard biochemical methods,can now be identified using this PCR assay. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/07/20 alle ore 07:07:26