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Titolo:
AUTOMATED MOLECULAR-GENETIC DNA ANALYSIS FOR DETECTING B-CELL NON-HODGKINS-LYMPHOMA IN CYTOLOGIC SPECIMENS
Autore:
RUSCHENBURG I; SCHLOTT T; LINKE B; REIMER S; DROESE M;
Indirizzi:
UNIV GOTTINGEN,DEPT PATHOL,DIV CYTOPATHOL,ROBERT KOCH STR 40 D-37075 GOTTINGEN GERMANY
Titolo Testata:
Analytical and quantitative cytology and histology
fascicolo: 3, volume: 19, anno: 1997,
pagine: 255 - 263
SICI:
0884-6812(1997)19:3<255:AMDAFD>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; FINE-NEEDLE ASPIRATION; CHROMOSOMAL TRANSLOCATION; FOLLICULAR HYPERPLASIA; KIEL CLASSIFICATION; STRUCTURAL-ANALYSIS; BCL-2 ONCOGENE; T(14-18); DIAGNOSIS; BIOPSY;
Keywords:
NON-HODGKINS LYMPHOMA; ASPIRATION BIOPSY; MOLECULAR GENETICS; POLYMERASE CHAIN REACTION; AUTOMATED DNA SEQUENCER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
I. Ruschenburg et al., "AUTOMATED MOLECULAR-GENETIC DNA ANALYSIS FOR DETECTING B-CELL NON-HODGKINS-LYMPHOMA IN CYTOLOGIC SPECIMENS", Analytical and quantitative cytology and histology, 19(3), 1997, pp. 255-263

Abstract

OBJECTIVE: In fine needle aspiration cytology, conventional morphologic and immunocytologic criteria are often insufficient for distinguishing the mixed type of follicular lymphoma (NHL)fram reactive lymphoid hyperplasia (RLH). Far improving cytodiagnosis, we adapted computer-assisted polymerase chain reaction (PCR) assays on cytologic specimens. These novel techniques aim at fluorescence-based sequencing and sizingof per products that result from NHL-specific markers, such as the chromosomal t(14;18) (q32;q21) translocation and the CDR3 regions of expanded B-cell neoplasia. STUDY DESIGN: Aspirates from eight cases with mixed-type follicular NHL and fi om Mine cases with a cytologic finding of RLH were investigated by PCR and with an automated laser scanningsystem. RESULTS: PCR detected the t(14;18) translocation in 5/8 B cell NHL samples and 1/9 RLH samples. By means of automated DNA sequencing, two of the five positive probes in the group of NHLs were identified as false positive. The CDR3 regions of expanded B-cell clones were found in four Of eight NHL aspirates by means of per and computer-assisted analysis. CONCLUSION: This study proved the reliability of automated genetic assays for diagnosing low grade B-cell NHLs in aspirates since false positive results are excluded.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 14:05:49