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Titolo:
The double-stranded RNA-binding protein Staufen is incorporated in human immunodeficiency virus type 1: Evidence for a role in genomic RNA encapsidation
Autore:
Mouland, AJ; Mercier, J; Luo, M; Bernier, L; DesGroseillers, L; Cohen, EA;
Indirizzi:
Univ Montreal, Human Retrovirol Lab, Dept Immunol & Microbiol, Montreal, PQ H3C 3J7, Canada Univ Montreal Montreal PQ Canada H3C 3J7 ol, Montreal, PQ H3C 3J7, Canada Univ Montreal, Dept Biochem, Montreal, PQ H3C 3J7, Canada Univ Montreal Montreal PQ Canada H3C 3J7 em, Montreal, PQ H3C 3J7, Canada
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 12, volume: 74, anno: 2000,
pagine: 5441 - 5451
SICI:
0022-538X(200006)74:12<5441:TDRPSI>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
ROUGH ENDOPLASMIC-RETICULUM; HIV-1 NUCLEOCAPSID PROTEIN; MESSENGER-RNA; IN-VIVO; HIPPOCAMPAL-NEURONS; SURFACE EXPRESSION; PARTICLE FORMATION; MAMMALIAN STAUFEN; GAG POLYPROTEIN; MATRIX PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
76
Recensione:
Indirizzi per estratti:
Indirizzo: Cohen, EA Univ Montreal, Human Retrovirol Lab, Dept Immunol & Microbiol, POB 6128,Stn Ctr Ville, Montreal, PQ H3C 3J7, Canada Univ Montreal POB 6128,Stn Ctr Ville Montreal PQ Canada H3C 3J7
Citazione:
A.J. Mouland et al., "The double-stranded RNA-binding protein Staufen is incorporated in human immunodeficiency virus type 1: Evidence for a role in genomic RNA encapsidation", J VIROLOGY, 74(12), 2000, pp. 5441-5451

Abstract

Human Staufen (hStau), a double-stranded RNA (dsRNA)-binding protein that is involved in mRNA transport, is incorporated in human immunodeficiency virus type 1 (HIV-1) and in other retroviruses, including HIV-2 and Moloney murine leukemia virus. Sucrose and Optiprep gradient analyses reveal cosedimentation of hStau with purified HIV-1, while subtilisin assays demonstrate that it is internalized. hStau incorporation in HIV-1 is selective, is dependent on an intact functional dsRNA-binding domain, and quantitatively correlates with levels of encapsidated HIV-1 genomic RNA. By coimmunoprecipitation and reverse transcription-PCR analyses, we demonstrate that hStau is associated with HIV-1 genomic RNA in HIV-1-expressing cells and purified virus, Overexpression of hStau enhances virion incorporation levels, and a corresponding, threefold increase in HIV-1 genomic RNA encapsidation levels. This coordinated increase in hStau and genomic RNA packaging had a significant negative effect on viral infectivity, This study is the first to describehStau within HIV-1 particles and provides evidence that hStau binds HIV-1 genomic RNA, indicating that it may be implicated in retroviral genome selection and packaging into assembling virions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 13:33:26