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Titolo:
CHARACTERIZATION OF 2 DISTINCT INTRACELLULAR GLUT4 MEMBRANE POPULATIONS IN MUSCLE-FIBER - DIFFERENTIAL PROTEIN-COMPOSITION AND SENSITIVITY TO INSULINS
Autore:
SEVILLA L; TOMAS E; MUNOZ P; GUMA A; FISCHER Y; THOMAS J; RUIZMONTASELL B; TESTAR X; PALACIN M; BLASI J; ZORZANO A;
Indirizzi:
UNIV BARCELONA,FAC BIOL,DEPT BIOQUIM & BIOL MOL,DIAGONAL 645 E-08028 BARCELONA SPAIN UNIV BARCELONA,FAC BIOL,DEPT BIOQUIM & BIOL MOL E-08028 BARCELONA SPAIN UNIV BARCELONA,DEPT BIOL CELLULAR & ANAT PATOL E-08028 BARCELONA SPAIN RHEIN WESTFAL TH AACHEN,FAC MED,INST PHYSIOL D-52057 AACHEN GERMANY
Titolo Testata:
Endocrinology
fascicolo: 7, volume: 138, anno: 1997,
pagine: 3006 - 3015
SICI:
0013-7227(1997)138:7<3006:CO2DIG>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT SKELETAL-MUSCLE; GLUCOSE-TRANSPORTER EXPRESSION; BROWN ADIPOSE-TISSUE; CELL-SURFACE; INDUCED TRANSLOCATION; GLUT4-ENRICHED VESICLES; SUBCELLULAR TRAFFICKING; PLASMA-MEMBRANE; WHITE MUSCLE; ADIPOCYTES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
62
Recensione:
Indirizzi per estratti:
Citazione:
L. Sevilla et al., "CHARACTERIZATION OF 2 DISTINCT INTRACELLULAR GLUT4 MEMBRANE POPULATIONS IN MUSCLE-FIBER - DIFFERENTIAL PROTEIN-COMPOSITION AND SENSITIVITY TO INSULINS", Endocrinology, 138(7), 1997, pp. 3006-3015

Abstract

A major objective For the understanding of muscle glucose disposal isthe elucidation of the intracellular trafficking pathway of GLUT4 glucose carriers in the muscle fiber. In this report, we provide functional and biochemical characterization of two distinct intracellular GLUT4 vesicle pools obtained from rat skeletal muscle. The two pools showed a differential response to insulin: thus, one showed a marked decrease in GLUT4 levels but the other did not. They also showed a markedly different protein composition as detected by quantitative vesicle immunoisolation analysis. The GLUT4 pool showing no response to insulin contained SCAMP proteins and the vSNARE proteins VAMP2 and cellubrevin. whereas only VAMPS was found in the insulin-recruitable GLUT4 pool. SDS-PAGE and further silver staining of the immunoprecipitates revealed discrete polypeptide bands associated to the insulin-sensitive pool, and all these polypeptide bands were found in the insulin-insensitive population. Furthermore, some polypeptide bands were exclusive to the insulin-insensitive population. The presence of cellubrevin and SCAMP proteins, endosomal markers, suggest that the insulin-insensitive GLUT4membrane population belongs to an. endosomal compartment. In addition, Re favor the view that the insulin-sensitive GLUT4 membrane pool is segregated from the endosomal GLUT4 population and is undergoes exocytosis to the cell surface in response to insulin. Intracellular GLUT4 membranes obtained from skeletal muscle contain cellubrevin, and VAMP2 and GLUT4-vesicles from cardiomyocytes also contain cellubrevin. This suggests that vSNARE proteins are key constituents of GLUT4 vesicles. The presence of the tSNARE protein SNAP25 in skeletal muscle membranesand SNAP25 and syntaxin 1A and syntaxin 1B in cardiomyocyte plasma membranes further suggest a role of the SNAREs in GLUT4 trafficking in muscle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 09:07:09