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Titolo:
A new method to visualize the Helicobacter pylori-associated Lewis(b)-binding adhesin utilizing SDS-digested freeze-fracture replica labeling
Autore:
Peterson, C; Larsson, B; Mahdavi, J; Boren, T; Magnusson, KE;
Indirizzi:
Linkoping Univ, Fac Hlth Sci, Div Med Microbiol, Dept Hlth & Environm, SE-58185 Linkoping, Sweden Linkoping Univ Linkoping Sweden SE-58185 onm, SE-58185 Linkoping, Sweden Umea Univ, Dept Odontol, Umea, Sweden Umea Univ Umea SwedenUmea Univ, Dept Odontol, Umea, Sweden
Titolo Testata:
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
fascicolo: 6, volume: 48, anno: 2000,
pagine: 877 - 883
SICI:
0022-1554(200006)48:6<877:ANMTVT>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTEGRAL MEMBRANE-PROTEINS; BLOOD-GROUP ANTIGENS; VACUOLATING CYTOTOXIN; NEISSERIA-GONORRHOEAE; ELECTRON-MICROSCOPY; ESCHERICHIA-COLI; DNA-BINDING; BLEBS;
Keywords:
freeze-fracture; Helicobacter pylori; SDS-digested freeze-fracture replica labeling; Lewis(b) blood group antigen-binding adhesin; BabA; bacterial envelopes; prokaryotic cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Peterson, C Linkoping Univ, Fac Hlth Sci, Div Med Microbiol, Dept Hlth & Environm, SE-58185 Linkoping, Sweden Linkoping Univ Linkoping Sweden SE-58185 5 Linkoping, Sweden
Citazione:
C. Peterson et al., "A new method to visualize the Helicobacter pylori-associated Lewis(b)-binding adhesin utilizing SDS-digested freeze-fracture replica labeling", J HIST CYTO, 48(6), 2000, pp. 877-883

Abstract

Freeze-fracture replica labeling has become a versatile tool to visualize both membrane components and other cell structures using SDS-replica cleaning before specific immunogold labeling of proteins or lipids. We report here for the first time the adoption and optimization of the method to studiesof bacterial envelopes, as applied to structural analysis of the distribution of the unique BabA-adhesin of the gastric pathogen Helicobacter pylori. BabA is important for bacterial adherence to the human epithelial cell lining of the stomach. The adhesin was found to be distributed all over the bacterial cell surfaces. Our results suggest that the SDS-replica labeling allows assessment of protein localization to distinct cell compartments and analysis of co-localization with neighboring membrane structures.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/08/20 alle ore 03:35:41