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Titolo:
Glibenclamide but not other sulphonylureas stimulates release of neuropeptide Y from perifused rat islets and hamster insulinoma cells
Autore:
Kulkarni, RN; Wang, ZL; Wang, RM; Smith, DM; Ghatei, MA; Bloom, SR;
Indirizzi:
Univ London Imperial Coll Sci Technol & Med, Sch Med, Dept Metab Med, Francis Fraser Labs, London W12 0NN, England Univ London Imperial Coll Sci Technol & Med London England W12 0NN gland
Titolo Testata:
JOURNAL OF ENDOCRINOLOGY
fascicolo: 2, volume: 165, anno: 2000,
pagine: 509 - 518
SICI:
0022-0795(200005)165:2<509:GBNOSS>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
PANCREATIC BETA-CELLS; DEXAMETHASONE TREATMENT; SULFONYLUREA RECEPTOR; PARACRINE ROLE; KDA PROTEIN; SECRETION; EXPRESSION; GLUCOSE; POLYPEPTIDE; PEPTIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Bloom, SR Univ London Imperial Coll Sci Technol & Med, Sch Med, Dept MetabMed, Francis Fraser Labs, Hammersmith Campus,Du Cane Rd, London W12 0NN, England Univ London Imperial Coll Sci Technol & Med Hammersmith Campus,Du Cane Rd London England W12 0NN
Citazione:
R.N. Kulkarni et al., "Glibenclamide but not other sulphonylureas stimulates release of neuropeptide Y from perifused rat islets and hamster insulinoma cells", J ENDOCR, 165(2), 2000, pp. 509-518

Abstract

We have studied the effects of first and second generation sulphonylureas on the release of insulin and neuropeptide tyrosine (NPY) fi-om hamster insulinoma tumour (HIT-T15) cells and isolated rat islets. In the presence of 5.5 mmol/l glucose all sulphonylureas stimulated insulin release from the HIT cells (P<0.01 ANOVA, n greater than or equal to 4) but only glibenclamide (GLIB, 10 mu mol/l) stimulated the release of NPY (mean +/- S.E.M. control 11.1 +/- 1.3 vs GLIB 28.4 +/- 4.1 fmol/h per 10(6) cells, P<0001, n=16). In isolated perifused rat islets both glibenclamide (10 mu mol/l) (control 3.5 +/- 0.3 vs GLIB 6.3 +/- 0.2 fmol/min per islet, P<0.01, n=6) and tolbutamide (50 mu mol/l) (control 4.7 +/- 0.1 vs TOLB 6.7 +/- 0.3 fmol/min per islet, P<0.01, n=6) enhanced glucose (8 mmol/l)-stimulated insulin release. However, only glibenclamide stimulated the release of NPY from the islets (control 3.4 +/- 0.8 vs GLIB 24.5 +/- 5 attomol/min per islet, P<0.01, n=6). Similar results were obtained in islets isolated from dexamethasone-treated rats. Glibenclamide treatment of HIT cells showed a prompt insulin release (10 min) while NPY secretion was slower (60 min), suggesting that internalization of the sulphonylurea is required to stimulate NPY release. Glibenclamide, the most common oral therapeutic agent in type 2 diabetes mellitus,is associated with release of the autocrine insulin secretion inhibitor, NPY.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 15:06:12