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Titolo:
Yeast nucleoporins involved in passive nuclear envelope permeability
Autore:
Shulga, N; Mosammaparast, N; Wozniak, R; Goldfarb, DS;
Indirizzi:
Univ Rochester, Dept Biol, Rochester, NY 14627 USA Univ Rochester Rochester NY USA 14627 Dept Biol, Rochester, NY 14627 USA Univ Alberta, Dept Cell Biol, Edmonton, AB T6G 2H7, Canada Univ Alberta Edmonton AB Canada T6G 2H7 iol, Edmonton, AB T6G 2H7, Canada
Titolo Testata:
JOURNAL OF CELL BIOLOGY
fascicolo: 5, volume: 149, anno: 2000,
pagine: 1027 - 1038
SICI:
0021-9525(20000529)149:5<1027:YNIIPN>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEMBRANE-PROTEIN POM152P; MESSENGER-RNA EXPORT; PORE COMPLEX; SACCHAROMYCES-CEREVISIAE; LOCALIZATION SIGNAL; TRANSPORT KINETICS; RAN-GTPASE; IMPORT; ARCHITECTURE; BINDING;
Keywords:
nuclear pore complex; import/export signals; green fluorescent protein; diffusion channel; Saccharomyces cerevisiae;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Goldfarb, DS Univ Rochester, Dept Biol, Rochester, NY 14627 USA Univ Rochester Rochester NY USA 14627 ochester, NY 14627 USA
Citazione:
N. Shulga et al., "Yeast nucleoporins involved in passive nuclear envelope permeability", J CELL BIOL, 149(5), 2000, pp. 1027-1038

Abstract

The vertebrate nuclear pore complex (NPC) harbors an similar to 10-nm diameter diffusion channel that is large enough to admit 50-kD polypeptides. Wehave analyzed the permeability properties of the Saccharomyces cerevisiae nuclear envelope (NE) using import (NLS) and export (NES) signal-containinggreen fluorescent protein (GFP) reporters. Compared with wild-type, passive export rates of a classical karyopherin/importin (Kap) Kap60p/Kap95p-targeted NLS-GFP reporter (cNLS-GFP) were significantly faster in nup188-Delta and nup170-Delta cells. Similar results were obtained using two other NLS-GFP reporters, containing either the kap104p-targeted Nab2p NLS (rgNLS) or the Kap121p-targeted Pho4p NLS (pNLS). Elevated levels of Hsp70 stimulated cNLS-GFP import, but had no effect on the import of rgNLS-GFP. Thus, the role of Hsp70 in NLS-directed import may be NLS- or targeting pathway-specific. Equilibrium sieving limits for the diffusion channel were assessed in vivo using NES-GFP reporters of 36-126 kD and were found to be greater than wild-type in nup188-Delta and nup170-Delta cells. We propose that Nup170p andNup188p are involved in establishing the functional resting diameter of the NPC's central transport channel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 13:13:14