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Titolo:
Role of COPI in phagosome maturation
Autore:
Botelho, RJ; Hackam, DJ; Schreiber, AD; Grinstein, S;
Indirizzi:
Hosp Sick Children, Cell Biol Programme, Toronto, ON M5G 1X8, Canada Hosp Sick Children Toronto ON Canada M5G 1X8 Toronto, ON M5G 1X8, Canada Univ Toronto, Dept Biochem, Toronto, ON M5G 1X8, Canada Univ Toronto Toronto ON Canada M5G 1X8 ochem, Toronto, ON M5G 1X8, Canada Univ Penn, Sch Med, Dept Med, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 Dept Med, Philadelphia, PA 19104 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 21, volume: 275, anno: 2000,
pagine: 15717 - 15727
SICI:
0021-9258(20000526)275:21<15717:ROCIPM>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR-MEDIATED PHAGOCYTOSIS; MYCOBACTERIUM-TUBERCULOSIS PHAGOSOME; MACROPHAGE FC-RECEPTORS; ADP-RIBOSYLATION FACTOR; VACUOLAR H+-ATPASE; BREFELDIN-A; MULTIVESICULAR ENDOSOMES; MEMBRANE-PROTEINS; EPSILON-COP; INTRACELLULAR TRAFFICKING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
71
Recensione:
Indirizzi per estratti:
Indirizzo: Botelho, RJ Div Cell Biol, 555 Univ Ave, Toronto, ON M5G 1X8, Canada Div Cell Biol 555 Univ Ave Toronto ON Canada M5G 1X8 8, Canada
Citazione:
R.J. Botelho et al., "Role of COPI in phagosome maturation", J BIOL CHEM, 275(21), 2000, pp. 15717-15727

Abstract

Phagosomes mature by sequentially fusing with endosomes and lysosomes. Vesicle budding is presumed to occur concomitantly, mediating the retrieval ofplasmalemmal components and the regulation of phagosomal size. We analyzedwhether fission of vesicles from phagosomes requires COPI, a multimeric complex known to be involved in budding from the Golgi and endosomes. The role of COPI was studied using ldlF cells, that harbor a temperature-sensitivemutation in E-COP, a subunit of the coatomer complex. These cells were made phagocytic toward IgG-opsonized particles by heterologous expression of human Fc gamma RIIA receptors. Following incubation at the restrictive temperature, epsilon-COP was degraded in these cells and their Golgi complex dispersed. Nevertheless, phagocytosis persisted for hours in cells devoid of epsilon-COP. Retrieval of transferrin receptors from phagosomes became inefficient in the absence of epsilon-COP, while clearance of the Fc gamma RIIA receptors was unaffected. This indicates that fission of vesicles from the phagosomal membrane involves at least two mechanisms, one of which requiresintact COPI. Traffic of fluid-phase markers and aggregated IgG-receptor complexes along the endocytic pathway was abnormal in epsilon-COP-deficient cells. In contrast, phagosome fusion with endosomes and lysosomes was unimpaired. Moreover, the resulting phagolysosomes were highly acidic. Similar results were obtained in RAW264.7 macrophages treated with brefeldin A, whichprecludes COPI assembly by interfering with the activation of adenosine ribosylation factor. These data indicate that neither phagosome formation normaturation are absolutely dependent on COPI. Our findings imply that phagosomal maturation differs from endosomal progression, which appears to be more dependent on COPI-mediated formation of carrier vesicles.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 00:15:06