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Titolo:
Construction of ELISA system to quantify human ST2 protein in sera of patients
Autore:
Kuroiwa, K; Li, HJ; Tago, K; Iwahana, H; Yanagisawa, K; Komatsu, N; Oshikawa, K; Sugiyama, Y; Arai, T; Tominaga, SI;
Indirizzi:
Jichi Med Sch, Dept Biochem, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 3290498 Tochigi 3290498, Japan Jichi Med Sch, Dept Med, Div Hematol, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 3290498 Tochigi 3290498, Japan Jichi Med Sch, Dept Pulm Med, Minami Kawachi, Tochigi 3290498, Japan JichiMed Sch Minami Kawachi Tochigi Japan 3290498 Tochigi 3290498, Japan Sci Univ Tokyo, Fac Sci & Technol, Dept Appl Biol Sci, Chiba 2788510, Japan Sci Univ Tokyo Chiba Japan 2788510 t Appl Biol Sci, Chiba 2788510, Japan
Titolo Testata:
HYBRIDOMA
fascicolo: 2, volume: 19, anno: 2000,
pagine: 151 - 159
SICI:
0272-457X(200004)19:2<151:COESTQ>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
HELPER T-CELLS; INTERLEUKIN-1 RECEPTOR; RESPONSIVE GENE; PROMOTER USAGE; MAST-CELLS; EXPRESSION; PRODUCT; TRANSCRIPTION; FIBROBLASTS; BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Tominaga, SI Jichi Med Sch, Dept Biochem, 3311-1 Yakushiji, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch 3311-1 Yakushiji Minami Kawachi Tochigi Japan 3290498
Citazione:
K. Kuroiwa et al., "Construction of ELISA system to quantify human ST2 protein in sera of patients", HYBRIDOMA, 19(2), 2000, pp. 151-159

Abstract

The human ST2 gene can be specifically induced by growth stimulation in fibroblastic cells, and can also be induced by antigen stimulation in Th2 cells. The gene encodes a soluble secreted protein, ST2, and a transmembrane protein, ST2L, which are closely related to the interleukin-l receptor. To gain insight into the biological roles of the ST2 gene, three monoclonal antibodies (MAbs) against human ST2 gene products were obtained. To obtain these antibodies, immunization was carried out using two different immunogens:purified soluble human ST2 protein (hST2), and COS7 cells, which express the extracellular portion of human ST2L, 2A5 and FB9 MAbs were derived from the immunization with soluble hST2, and HB12 was derived from the COS7 cellimmunization. All three antibodies were shown to detect native forms of the human ST2 gene products by immunoprecipitation, flow cytometry, and enzyme-linked immunosorbent assay (ELISA). In the competitive ELISA using biotinylated and nonlabelled MAbs, neither FB9 nor HB12 affected the binding of 2A5 to ST2 gene products. Based on this result, we constructed a sandwich ELISA system using 2A5 and FB9 to measure the concentration of soluble hST2 in sera. The ELISA, combined with the flow cytometry using these antibodies,will be a useful tool for elucidating the functions of human ST2 gene products in individuals.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 12:51:00