Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Excess of glucocorticoids impairs whole-body antioxidant status in young rats. Relation to the effect of dexamethasone in soleus muscle and spleen
Autore:
Orzechowski, A; Ostaszewski, P; Brodnicka, A; Wilczak, J; Jank, M; Balasinska, B; Grzelkowska, K; Ploszaj, T; Olczak, J; Mrowczynska, A;
Indirizzi:
Agr Univ Warsaw, Fac Vet Med, Dept Physiol Biochem Pharmacol & Toxicol, PL-02785 Warsaw, Poland Agr Univ Warsaw Warsaw Poland PL-02785 Toxicol, PL-02785 Warsaw, Poland
Titolo Testata:
HORMONE AND METABOLIC RESEARCH
fascicolo: 5, volume: 32, anno: 2000,
pagine: 174 - 180
SICI:
0018-5043(200005)32:5<174:EOGIWA>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUPEROXIDE-DISMUTASE; OXIDATIVE STRESS; SKELETAL-MUSCLE; CELL-DEATH; OLD RATS; INSULIN-RESISTANCE; APOPTOSIS; GLUTATHIONE; DEFENSE; ADULT;
Keywords:
dexamethasone; oxidative stress; M. soleus; spleen; rat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Orzechowski, A Agr Univ Warsaw, Fac Vet Med, Dept Physiol Biochem Pharmacol & Toxicol, Nowoursynowska 166, PL-02785 Warsaw, Poland Agr Univ Warsaw Nowoursynowska 166 Warsaw Poland PL-02785
Citazione:
A. Orzechowski et al., "Excess of glucocorticoids impairs whole-body antioxidant status in young rats. Relation to the effect of dexamethasone in soleus muscle and spleen", HORMONE MET, 32(5), 2000, pp. 174-180

Abstract

The action of glucocorticoids in high doses is catabolic, but not much is known about the accompanying effects on antioxidative capacity of the entire body. Animals were treated (or not) with dexamethasone (Dex) 2 mg/kg b.w.d(-1) during 5 consecutive days followed by recovery, during which an additional group received 3-hydroxy-3-methylbutyrate (40 mg/kg b.w). Animals were killed after treatment with Dex, and after 5 days of the recovery period. Dexamethasone treatment decreased appetite almost twofold (from 20 g/day to 10 g/day, P < 0.001). Feed restriction, however, seemed to have only minor impact on the effects observed since body weight loss of pair-fed rats after the 5th day of treatment was only 2 % and Dex-treated rats decrease inbody weight was 22% (P < 0.05). In turn, wet weight of the soleus muscle (expressed per body weight) did not significantly decrease after Dex treatment, suggesting relative resistance of oxidative type muscles to the catabolic action of dexamethasone. Spleen wet weight expressed per body weight dropped by 65% (P<0.001). Additionally, there was a 46% reduction (P<0.007) ofblood glutathione (GSH/Hb), and 36% (P< 0.001) of muscle glutathione (GSH/tissue wet weight). This suggests that dexamethasone directly and/or indirectly impaired antioxidant reactions. This was further confirmed by a significant (49 %) decline of SOD-l activity in erythrocytes isolated from the group treated with dexamethasone. Another index of lipid peroxidation (TBARS)was also significantly increased. Activity of blood plasma CK increased by73% (P<0.001) in Dex-treated rats, indicating moderate injury of muscle tissue. in conclusion, young growing rats were sensitive to the dosage of dexamethasone, but in contrast to lymphoid tissue, could easily compensate theoutcomes of impaired antioxidative defence within 5 days of recovery.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 23:24:56