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Titolo:
Experimental subretinal neovascularization is inhibited by adenovirus-mediated soluble VEGF/flt-1 receptor gene transfection: a role of VEGF and possible treatment for SRN in age-related macular degeneration
Autore:
Honda, M; Sakamoto, T; Ishibashi, T; Inomata, H; Ueno, H;
Indirizzi:
Kyushu Univ, Fac Med, Dept Ophthalmol, Higashi Ku, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 ol, Higashi Ku, Fukuoka 8128582, Japan Kyushu Univ, Fac Med, Res Inst Angiocardiol & Cardiovasc Clin, Dept Mol Cardiol, Fukuoka 812, Japan Kyushu Univ Fukuoka Japan 812 Clin, Dept Mol Cardiol, Fukuoka 812, Japan
Titolo Testata:
GENE THERAPY
fascicolo: 11, volume: 7, anno: 2000,
pagine: 978 - 985
SICI:
0969-7128(200006)7:11<978:ESNIIB>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIAL GROWTH-FACTOR; PIGMENT EPITHELIAL-CELLS; EXPERIMENTAL CHOROIDAL NEOVASCULARIZATION; IN-VITRO; EXPRESSION; RAT; ANGIOGENESIS; MODEL; SUPPRESSION; MEMBRANES;
Keywords:
gene transfection; gene therapy; subretinal neovascularization; age-related macular degeneration;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Sakamoto, T Kyushu Univ, Fac Med, Dept Ophthalmol, Higashi Ku, 3-1-1 Maidashi, Fukuoka8128582, Japan Kyushu Univ 3-1-1 Maidashi Fukuoka Japan 8128582 28582, Japan
Citazione:
M. Honda et al., "Experimental subretinal neovascularization is inhibited by adenovirus-mediated soluble VEGF/flt-1 receptor gene transfection: a role of VEGF and possible treatment for SRN in age-related macular degeneration", GENE THER, 7(11), 2000, pp. 978-985

Abstract

Accumulating evidence has shown the importance of vascular endothelial growth factor (VEGF) in chorioretinal angiogenesis. However, whether or not VEGF is indeed critical for the pathogenesis of subretinal neovascularization(SRN) in adulthood, which is a serious complication of age-related maculardegeneration, has to be further investigated We constructed an adenovirus expressing an entire ectodomain of the human VEGF receptor/flt-1 fused to Fc portion of human IgG (Adflt-ExR): this soluble receptor is secreted from Adflt-ExR-transfected cells. We studied the effect of Adflt-ExR on the formation of experimental SRN. Experimental SRN was induced by intense photocoagulation on the retina in pigmented rats and Adflt-ExR was injected into the femoral muscle. The formation of SRN assessed by fluorescein angiography was more significantly inhibited for 7 days in the Adflt-ExR-treated rats than in the control rats who received either an adenovirus vector encoding LacZ gene or balanced salt solution (BSS). The serum concentration of this soluble receptor increased for 7 days and thereafter gradually decreased. Animmunohistochemical study disclosed the fibroblast cell proliferation and inflammatory cell infiltration to be reduced in the photocoagulation spot of Adflt-ExR-treated rats. VEGF plays a crucial role in the formation of SRNand VEGF soluble receptor gene transfection can inhibit SRN. This method will contribute to future gene therapy for age-related macular degeneration.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 07:01:06