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Titolo:
Isolation and characterization of the epothilone biosynthetic gene clusterfrom Sorangium cellulosum
Autore:
Julien, B; Shah, S; Ziermann, R; Goldman, R; Katz, L; Khosla, C;
Indirizzi:
Kosan Biosci Inc, Hayward, CA 94545 USA Kosan Biosci Inc Hayward CA USA 94545 n Biosci Inc, Hayward, CA 94545 USA
Titolo Testata:
GENE
fascicolo: 1-2, volume: 249, anno: 2000,
pagine: 153 - 160
SICI:
0378-1119(20000516)249:1-2<153:IACOTE>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICROTUBULE-STABILIZING AGENTS; POLYKETIDE SYNTHASE GENES; PEPTIDE SYNTHETASES; ESCHERICHIA-COLI; STREPTOMYCES; DNA; ORGANIZATION; SPECIFICITY; DIVERSITY; CLONING;
Keywords:
myxobacteria; non-ribosomal peptide synthetase; polyketide; polyketide synthase; P450 enzyme;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Julien, B Kosan Biosci Inc, 3832 Bay Ctr Pl, Hayward, CA 94545 USA Kosan Biosci Inc 3832 Bay Ctr Pl Hayward CA USA 94545 94545 USA
Citazione:
B. Julien et al., "Isolation and characterization of the epothilone biosynthetic gene clusterfrom Sorangium cellulosum", GENE, 249(1-2), 2000, pp. 153-160

Abstract

The epothilone biosynthetic gene cluster was isolated from Sorangium cellulosum strain SMP44. The gene cluster contains seven genes and spans approx.56 kb. The genes encoding the PKS, epoA, epoC, epoD, epoE, and epoF, are divided into nine modules. The EpoB protein is a non-ribosomal peptide synthetase (NRPS) that catalyzes formation of the thiazole found in the epothilones. EpoK is a P450 enzyme responsible for the epoxidation of epothilones Cand D to epothilones A and B, respectively. EpoK was expressed in Escherichia coli, and the purified protein was shown to convert epothilone D to epothilone B in vitro. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 15:46:35