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Titolo:
Regulation of secretory protein gene expression in Paramecium - Role of the cortical exocytotic sites
Autore:
Galvani, A; Sperling, L;
Indirizzi:
CNRS, Ctr Genet Mol, F-91198 Gif Sur Yvette, France CNRS Gif Sur Yvette France F-91198 t Mol, F-91198 Gif Sur Yvette, France
Titolo Testata:
EUROPEAN JOURNAL OF BIOCHEMISTRY
fascicolo: 11, volume: 267, anno: 2000,
pagine: 3226 - 3234
SICI:
0014-2956(200006)267:11<3226:ROSPGE>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
NF-KAPPA-B; SYNCHRONOUS EXOCYTOSIS; MEMBRANE-FUSION; PHEOCHROMOCYTOMA CELLS; CA2+ INFLUX; CALCIUM; TRANSCRIPTION; TETRAURELIA; ACTIVATION; PATHWAYS;
Keywords:
exocytosis; Paramecium; secretory mutants; transcription; trichocyst;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Sperling, L CNRS, Ctr Genet Mol, F-91198 Gif Sur Yvette, France CNRS Gif Sur Yvette France F-91198 98 Gif Sur Yvette, France
Citazione:
A. Galvani e L. Sperling, "Regulation of secretory protein gene expression in Paramecium - Role of the cortical exocytotic sites", EUR J BIOCH, 267(11), 2000, pp. 3226-3234

Abstract

In cells that possess a regulated secretory pathway, exocytosis can lead to transcriptional activation of genes encoding products stored in secretorygranules as well as genes required for granule biogenesis. With the objective of understanding this response, we have examined the expression of Paramecium secretory protein genes in different physiological and genetic contexts. The genes belong to the trichocyst matrix protein (TMP) multigene family, encoding polypeptides that form the crystalline matrix of the secretorygranules, known as trichocysts. Approximately 1000 trichocysts per cell are docked at pre-formed cortical exocytotic sites. Their rapid and synchronous exocytosis can be triggered by vital secretagogues such as aminoethyldextran without harming the cells. Using this exocytotic trigger, we found that the transcription of TMP genes undergoes rapid, transient and co-ordinate10-fold activation in response to massive exocytosis, leading to a 2.5-fold increase in the pool of TMP mRNA. Experiments with exocytosis-deficient mutants show that the secretagogue-induced increase in intracellular free calcium implicated in stimulus/secretion coupling is not sufficient to activate TMP gene expression. We present evidence that the state of occupation ofthe cortical exocytotic sites can affect TMP gene expression and suggest that these sites play a role in gene activation in response to exocytosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 22:16:12