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Titolo:
Injection of DNA encoding granulocyte-macrophage colony-stimulating factorrecruits dendritic cells for immune adjuvant effects
Autore:
Bowne, WB; Wolchok, JD; Hawkins, WG; Srinivasan, R; Gregor, P; Blachere, NE; Moroi, Y; Engelhorn, ME; Houghton, AN; Lewis, JJ;
Indirizzi:
Mem Sloan Kettering Canc Ctr, Swim Across Amer Lab, New York, NY 10021 USAMem Sloan Kettering Canc Ctr New York NY USA 10021 New York, NY 10021 USA
Titolo Testata:
CYTOKINES CELLULAR & MOLECULAR THERAPY
fascicolo: 4, volume: 5, anno: 1999,
pagine: 217 - 225
SICI:
1368-4736(199912)5:4<217:IODEGC>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMAL LANGERHANS CELLS; ENHANCED TUMOR PROTECTION; GM-CSF GENE; ANTITUMOR IMMUNITY; FACTOR EXPRESSION; IMMUNIZATION; VACCINES; RESPONSES; POTENT; AUTOIMMUNITY;
Keywords:
GM-CSF; DNA; vaccine; adjuvant;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Houghton, AN Mem Sloan Kettering Canc Ctr, Swim Across Amer Lab, 1275 YorkAve, New York, NY 10021 USA Mem Sloan Kettering Canc Ctr 1275 York Ave NewYork NY USA 10021
Citazione:
W.B. Bowne et al., "Injection of DNA encoding granulocyte-macrophage colony-stimulating factorrecruits dendritic cells for immune adjuvant effects", CYTOK CELL, 5(4), 1999, pp. 217-225

Abstract

An important issue for effective vaccines is the development of potent adjuvants that can facilitate induction or augmentation of immunity. Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a growth factor for myeloid progenitors of monocytes and dendritic cells (DC), which upon maturation are antigen-presenting cells (APC). The adjuvant effects of inoculation of DNA encoding GMCSF into skin were studied. Initial experiments examined whether the GM-CSF gene injected into the skin of mice could affect the density of epidermal DC (Langerhans cells). DNA encoding GM-CSF delivered by particle bombardment into skin resulted in a significant increase of epidermal DC at the inoculation site. kinetic analysis of epidermal recruitment after GM-CSF inoculation showed an increase in DC that peaked at seven days. This increase was accompanied by recruitment of DC into draining lymph nodes. The adjuvant effects of DNA encoding GM-CSF inoculated into skin were measured by the ability to augment antibody and T-cell responses against poorly immunogenic tumor antigens. Peptide immunization at skin sites containing epidermal DC newly recruited by GM-CSF DNA elicited T-cell responses against mutant p53, whereas peptide immunization of control skin sites did not elicit any detectable T-cell responses. Likewise, generation of antibodies following immunization with DNA encoding human gp75(TRP1), a tyrosinase family member expressed by melanomas, was accelerated and protection from tumor challenge augmented by GM-CSF DNA.

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Documento generato il 30/11/20 alle ore 16:40:08