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Titolo:
Synergy in induction of increased renal allograft survival after portal vein infusion of dendritic cells transduced to express TGF beta and IL-10, along with administration of CHO cells expressing the regulatory molecule OX-2
Autore:
Gorczynski, RM; Bransom, J; Cattral, M; Huang, X; Lei, J; Xiaorong, L; Min, WP; Wan, Y; Gauldie, J;
Indirizzi:
Toronto Hosp, Transplant Res Div, Toronto, ON M5T 2S8, Canada Toronto Hosp Toronto ON Canada M5T 2S8 s Div, Toronto, ON M5T 2S8, Canada Univ Toronto, Toronto, ON, Canada Univ Toronto Toronto ON CanadaUniv Toronto, Toronto, ON, Canada McMaster Univ, Dept Pathol, Hamilton, ON, Canada McMaster Univ Hamilton ON Canada Univ, Dept Pathol, Hamilton, ON, Canada
Titolo Testata:
CLINICAL IMMUNOLOGY
fascicolo: 3, volume: 95, anno: 2000,
pagine: 182 - 189
SICI:
1521-6616(200006)95:3<182:SIIOIR>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTIGEN-PRESENTING CELLS; IN-VIVO; FAS-LIGAND; VENOUS IMMUNIZATION; BONE-MARROW; IFN-GAMMA; MONOCLONAL-ANTIBODIES; GRAFT PROLONGATION; ALLOGENEIC CELLS; SKIN ALLOGRAFTS;
Keywords:
transplantation; immunoregulation; dendritic cells; cytokines; OX-2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Gorczynski, RM Toronto Hosp, Transplant Res Div, Toronto, ON M5T 2S8, Canada Toronto Hosp Toronto ON Canada M5T 2S8 ON M5T 2S8, Canada
Citazione:
R.M. Gorczynski et al., "Synergy in induction of increased renal allograft survival after portal vein infusion of dendritic cells transduced to express TGF beta and IL-10, along with administration of CHO cells expressing the regulatory molecule OX-2", CLIN IMMUNO, 95(3), 2000, pp. 182-189

Abstract

Dendritic cells (DC), generated from C57BL/6 mouse bone marrow cells cultured with GM-CSF and IL-4 for 9 days, were engineered to express constitutively the cytokines TGF beta, IL-10, and IL-12, using adenovirus vectors constructed using an E1-deleted replication-deficient recombinant adenovirus carrying the appropriate cDNA for the relevant cytokines (Ad-TGF beta, Ad-IL-12, or Ad-IL-10). C3H mice receiving nontransduced DC or pretransplant infusion of DC-Ad-LacZ showed increased survival of C57BL/6 renal grafts relative to that of control nonimmunized mice. Transfusion of Ad-IL-12-transducedDC abolished this increased survival, leading to a graft survival equivalent to that of controls with no DC. Optimal graft survival was seen in the group receiving a mixture of DC transduced with constructs for both IL-10 and TGF beta. There was a correlation between increased graft survival and both inhibition of the induction of CTL and enhancement of a polarization to produce type-2 cytokines (IL-4, IL-10, and TGF beta) on antigen-specific restimulation in vitro. These effects were more pronounced following concomitant infusion of CRO cells transfected with a full-length cDNA for murine OX-2. (C) 2000 Academic Press.

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Documento generato il 06/12/20 alle ore 00:02:10