Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The macrocyclic peptide antibiotic micrococcin P-1 is secreted by the food-borne bacterium Staphylococcus equorum WS 2733 and inhibits Listeria monocytogenes on soft cheese
Autore:
Carnio, MC; Holtzel, A; Rudolf, M; Henle, T; Jung, G; Scherer, S;
Indirizzi:
Tech Univ Munich, Inst Mikrobiol, FML Weihen Stephan, D-85354 Freising, Germany Tech Univ Munich Freising Germany D-85354 han, D-85354 Freising, Germany Univ Tubingen, Inst Organ Chem, D-72076 Tubingen, Germany Univ Tubingen Tubingen Germany D-72076 n Chem, D-72076 Tubingen, Germany Tech Univ Dresden, Inst Lebensmittelchem, D-01062 Dresden, Germany Tech Univ Dresden Dresden Germany D-01062 chem, D-01062 Dresden, Germany
Titolo Testata:
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
fascicolo: 6, volume: 66, anno: 2000,
pagine: 2378 - 2384
SICI:
0099-2240(200006)66:6<2378:TMPAMP>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
RED SMEAR CHEESE; BREVIBACTERIUM LINENS; CAMEMBERT CHEESE; PEDIOCOCCUS-ACIDILACTICI; CORYNEFORM BACTERIA; PROTEIN-SYNTHESIS; MANCHEGO CHEESE; RIPENING FLORA; IDENTIFICATION; GROWTH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Scherer, S Tech Univ Munich, Inst Mikrobiol, FML Weihen Stephan, D-85354 Freising, Germany Tech Univ Munich Freising Germany D-85354 4 Freising, Germany
Citazione:
M.C. Carnio et al., "The macrocyclic peptide antibiotic micrococcin P-1 is secreted by the food-borne bacterium Staphylococcus equorum WS 2733 and inhibits Listeria monocytogenes on soft cheese", APPL ENVIR, 66(6), 2000, pp. 2378-2384

Abstract

Staphylococcus equorum WS 2733 was found to produce a substance exhibitinga bacteriostatic effect on a variety of gram-positive bacteria. The metabolite was purified to homogeneity by ammonium sulfate precipitation and semipreparative reversed-phase high-performance liquid chromatography. Electrospray mass spectrometry confirmed the high purity of the compound and revealed a molecular mass of 1,143 Da, By two-dimensional nuclear magnetic resonance spectroscopy the substance was identified as micrococcin P-1 which is amacrocyclic peptide antibiotic that has not yet been reported for the genus Staphylococcus. A total of 95 out of 95 Listeria strains and 130 out of 135 other gram-positive bacteria were inhibited by this substance, while none of 37 gram-negative bacteria were affected. The antilisterial potential of this food-grade strain as a protective starter culture was evaluated by its in situ application in cheese-ripening experiments under laboratory conditions. A remarkable growth reduction of Listeria monocytogenes could be achieved compared to control cheese ripened with a nonbacteriocinogenic type strain of Staphylococus equorum, In order to prove that inhibition was due to micrococcin P-1, a micrococcin-deficient mutant was constructed which did not inhibit L. monocytogenes in cheese-ripening experiments.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 21:26:19