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Titolo:
Dynamic changes of intracellular pH in individual lactic acid bacterium cells in response to a rapid drop in extracellular pH
Autore:
Siegumfeldt, H; Rechinger, KB; Jakobsen, M;
Indirizzi:
Royal Vet & Agr Univ, Dept Dairy & Food Sci Food Microbiol, Frederiksberg 1958 C, Denmark Royal Vet & Agr Univ Frederiksberg Denmark 1958 C ksberg 1958 C, Denmark
Titolo Testata:
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
fascicolo: 6, volume: 66, anno: 2000,
pagine: 2330 - 2335
SICI:
0099-2240(200006)66:6<2330:DCOIPI>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTON-TRANSLOCATING ATPASE; CYTOPLASMIC PH; LACTOCOCCUS-LACTIS; LACTOBACILLUS-PLANTARUM; INTERNAL PH; SACCHAROMYCES-CEREVISIAE; SALMONELLA-TYPHIMURIUM; STREPTOCOCCUS-FAECALIS; FERMENTATION ACIDS; HOMEOSTASIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Siegumfeldt, H Royal Vet & Agr Univ, Dept Dairy & Food Sci Food Microbiol,Rolighedsvej 30, Frederiksberg 1958 C, Denmark Royal Vet & Agr Univ Rolighedsvej 30 Frederiksberg Denmark 1958 C
Citazione:
H. Siegumfeldt et al., "Dynamic changes of intracellular pH in individual lactic acid bacterium cells in response to a rapid drop in extracellular pH", APPL ENVIR, 66(6), 2000, pp. 2330-2335

Abstract

We describe the dynamics of changes in the intracellular pH (pH(i)) valuesof a number of lactic acid bacteria in response to a rapid drop in the extracellular pH (pH(ex)). Strains of Lactobacillus delbrueckii subsp. bulgaricus, Streptococcus thermophilus, and Lactococcus lactis were investigated. Listeria innocua, a gram-positive, non-lactic acid bacterium, was included for comparison. The method which we used was based on fluorescence ratio imaging of single cells, and it was therefore possible to describe variationsin pH(i) within a population, The bacteria were immobilized on a membrane filter, placed in a closed perfusion chamber, and analyzed during a rapid decrease in the pH(ex) from 7.0 to 5.0, Under these conditions, the pH(i) ofL. innocua remained neutral (between 7 and 8), In contrast, the pH(i) values of all of the strains of lactic acid bacteria investigated decreased to approximately 5.5 as the pH(ex) was decreased. No pronounced differences were observed between cells of the same strain harvested from the exponentialand stationary phases. Small differences between species were observed with regard to the initial pH(i) at pH(ex) 7.0, while different kinetics of pH(i) regulation were observed in different species and also in different strains of S. thermophilus.

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Documento generato il 10/04/20 alle ore 02:36:17