Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Interactions of HIV-1 Nef with the mu subunits of adaptor protein complexes 1, 2, and 3: Role of the dileucine-based sorting motif
Autore:
Craig, HM; Reddy, TR; Riggs, NL; Dao, PP; Guatelli, JC;
Indirizzi:
Univ Calif San Diego, Dept Pathol, San Diego, CA 92103 USA Univ Calif San Diego San Diego CA USA 92103 thol, San Diego, CA 92103 USA Univ Calif San Diego, Dept Med, San Diego, CA 92103 USA Univ Calif San Diego San Diego CA USA 92103 Med, San Diego, CA 92103 USA San Diego Vet Affairs Med Ctr, La Jolla, CA 92093 USA San Diego Vet Affairs Med Ctr La Jolla CA USA 92093 a Jolla, CA 92093 USA
Titolo Testata:
VIROLOGY
fascicolo: 1, volume: 271, anno: 2000,
pagine: 9 - 17
SICI:
0042-6822(20000525)271:1<9:IOHNWT>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; CD4 DOWN-REGULATION; COATED VESICLE PROTEINS; AP-3 ADAPTER; CLATHRIN ADAPTER; MEDIUM CHAINS; SURFACE CD4; I MOLECULES; BETA-COP; SIGNALS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Guatelli, JC Univ Calif San Diego, Dept Pathol, San Diego, CA 92103 USA Univ Calif San Diego San Diego CA USA 92103 go, CA 92103 USA
Citazione:
H.M. Craig et al., "Interactions of HIV-1 Nef with the mu subunits of adaptor protein complexes 1, 2, and 3: Role of the dileucine-based sorting motif", VIROLOGY, 271(1), 2000, pp. 9-17

Abstract

HIV-1 Nef interacts with cellular adaptor protein (AP) complexes and theirmedium (mu) subunits. However, the role of the dileucine-based sorting motif within Nef in these interactions has been incompletely characterized. Here, yeast two-hybrid assays indicated that HIV-1 Nef interacted not only with the mu subunits of AP-1 and AP-2, but also with that of AP-3. The interactions with mu 1 and mu 3 were markedly stronger than the interaction with mu 2. Leucine residues of the sorting motif were required for the interactions with mu 3 and mu 2 and contributed to the interaction with mu 1. Confocal immunofluorescence microscopy indicated that Nef, AP-I, and AP-3 (but not AP-2) were concentrated in a juxtanuclear region near the cell center, potentially facilitating interaction between Nei and the mu 1 and mu 3 subunits. However, leucine residues of the sorting motif were not required for this subcellular localization of Nef. These data suggest that the dileucine motif, required for optimal viral replication, functions through interactions with a variety of AP complexes, including AP-3, potentially by recruitingadaptor complexes to subcellular locations specified by additional determinants in the Nef protein. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 14:22:42