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Titolo:
Transplantation of human peripheral blood stem cells into fetal rhesus monkeys (Macaca mulatta)
Autore:
Tarantal, AF; Goldstein, O; Barley, F; Cowan, MJ;
Indirizzi:
Univ Calif Davis, Calif Reg Primate Res Ctr, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 Primate Res Ctr, Davis, CA 95616 USA Univ Calif Davis, Dept Pediat, Davis, CA 95616 USA Univ Calif Davis DavisCA USA 95616 vis, Dept Pediat, Davis, CA 95616 USA Univ Calif San Francisco, Pediat Bone Marrow Transplant Div, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA
Titolo Testata:
TRANSPLANTATION
fascicolo: 9, volume: 69, anno: 2000,
pagine: 1818 - 1823
SICI:
0041-1337(20000515)69:9<1818:TOHPBS>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-UTERO TRANSPLANTATION; INUTERO TRANSPLANTATION; MARROW TRANSPLANTATION; IMMUNODEFICIENT MICE; HEMATOPOIETIC-CELLS; FASCICULARIS; ENGRAFTMENT; ULTRASOUND; CYNOMOLGUS; MACAQUES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Tarantal, AF Univ Calif Davis, Calif Reg Primate Res Ctr, Pedrick & Hutchisons Rd, Davis, CA 95616 USA Univ Calif Davis Pedrick & Hutchisons Rd DavisCA USA 95616 A
Citazione:
A.F. Tarantal et al., "Transplantation of human peripheral blood stem cells into fetal rhesus monkeys (Macaca mulatta)", TRANSPLANT, 69(9), 2000, pp. 1818-1823

Abstract

Background. Methods for assessing engraftment efficiency have been explored in a primate xenogeneic model of in utero hematopoietic stem cell transplantation, Methods, Human peripheral blood stem cells (PBSC) were obtained by leukapheresis from a human male donor after 4 days of administration of recombinant human granulocyte-colony stimulating factor (5 mu g/kg/day). PBSC were enriched for the CD34(+) population with and without T-cell depletion. The resulting mononuclear cells consisted of two cell populations, one that was stem cell enriched (0.83% CD3(+) cells, 95% CD34(+); group 1) and one that was stem cell enriched and T-cell depleted (<0,03% CD3(+) cells, 98% CD34(+); group 2), Four fetal monkeys (two per group) received either twoor four i,p, injections (similar to 5x10(6) cells/injection) via ultrasound guidance every other day over a 7-day period (gestational days 50, 52, 54, and 56), One fetus in each group also received i.p. recombinant human stem cell factor (25 mu g/kg) and recombinant human granulocyte-colony stimulating factor (10 mu g/kg) posttransplant every 10 days from gestational day 60-150. Results. Four healthy newborns were delivered at term, and specimens were analyzed by polymerase chain reaction for the human Y chromosome (birth, monthly to 6 months; blood, marrow, progenitor assays). Polymerase chain reaction results were positive for all four newborns in all specimens assessed, and flow cytometric analysis for human CD45 in marrow showed engraftment ranging from 0.1-1,7%. There was no evidence of graft-versus-host disease in any of the animals. Conclusion. These studies show that (1) multilineage engraftment of human PBSC can be achieved in the fetal rhesus recipient, (2) the rhesus fetus appears to tolerate relatively high numbers of human CD3(+) cells, and (3) healthy chimeric rhesus infants can be delivered at term after multiple in utero procedures.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 18:55:36