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Titolo:
Simultaneous determination of human plasma levels of four selective serotonin reuptake inhibitors by high-performance liquid chromatography
Autore:
Lucca, A; Gentilini, G; Lopez-Silva, S; Soldarini, A;
Indirizzi:
Univ Milan, Osped San Raffaele, Ist Sci, Dept Neuropsychiat Sci, I-20127 Milan, Italy Univ Milan Milan Italy I-20127 t Neuropsychiat Sci, I-20127 Milan, Italy Dept Lab Med, Milan, Italy Dept Lab Med Milan ItalyDept Lab Med, Milan, Italy
Titolo Testata:
THERAPEUTIC DRUG MONITORING
fascicolo: 3, volume: 22, anno: 2000,
pagine: 271 - 276
SICI:
0163-4356(200006)22:3<271:SDOHPL>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
ULTRAVIOLET DETECTION; FLUVOXAMINE; PHARMACOKINETICS; NORFLUOXETINE; FLUORESCENCE; METABOLISM; FLUOXETINE;
Keywords:
selective serotonin reuptake inhibitors; HPLC; therapeutic drug monitoring;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Lucca, A Univ Milan, Osped San Raffaele, Ist Sci, Dept Neuropsychiat Sci, Via Prinetti 29, I-20127 Milan, Italy Univ Milan Via Prinetti 29 Milan Italy I-20127 0127 Milan, Italy
Citazione:
A. Lucca et al., "Simultaneous determination of human plasma levels of four selective serotonin reuptake inhibitors by high-performance liquid chromatography", THER DRUG M, 22(3), 2000, pp. 271-276

Abstract

A reversed-phase high-performance liquid chromatography (HPLC) method withfluorimetric detection, which allows the simultaneous determination of plasma concentrations of foul selective serotonin reuptake inhibitors (SSRIs) is presented. Fluvoxamine, paroxetine, sertraline, and fluoxetine were extracted from plasma with ethyl acetate and then derivatized with dansyl chloride. The analytes were separated using Hypersyl ODS C18 (5 mu m) 250 x 4.6 mm column (ThermoQuest, Runcorn, UK). For continuous gradient separation, the mobile phase consists of two eluents, acetonitrile and potassium phosphate buffer (10 mmol/L, pH 7.2) at total flow rate of 1.5 mL/min. Detection was carried out at lambda exc = 366 nm and lambda em = 490 nm. The authors found recoveries of 90% to 95% for fluvoxamine, 94% to 100% for paroxetine, 88% to 95% for sertraline, 93% to 100% for fluoxetine, and 97% to 100% for internal standard (nortriptyline). Imprecision of the method ranged from 2.5% to 8.9%. The assay was linear from 10 to 1500 ng/mL for sertraline, and from 5 to 1500 ng/mL for the other drugs. The authors conclude that this method is suitable for monitoring antidepressant therapy. In addition, the authors report the effects of adding paroxetine to fluvoxamine on plasma levels in a group of patients in combined drug therapy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/20 alle ore 07:44:56