Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Purification and characterisation of vanadium haloperoxidases from the brown alga Pelvetia canaliculata
Autore:
Almeida, MG; Humanes, M; Melo, R; Silva, JA; da Silva, JJRF; Wever, R;
Indirizzi:
Inst Super Tecn, Ctr Quim Estrutural, P-1049001 Lisbon, Portugal Inst Super Tecn Lisbon Portugal P-1049001 al, P-1049001 Lisbon, Portugal Univ Lisbon, Fac Ciencias, Dept Quim & Bioquim, Ctr Electroquim & Cinet, P-1749016 Lisbon, Portugal Univ Lisbon Lisbon Portugal P-1749016 Cinet, P-1749016 Lisbon, Portugal Univ Lisbon, Fac Ciencias, Inst Oceanog, P-1749016 Lisbon, Portugal Univ Lisbon Lisbon Portugal P-1749016 ceanog, P-1749016 Lisbon, Portugal Univ Amsterdam, EC Slater Inst Biochem Res, NL-1018 TV Amsterdam, Netherlands Univ Amsterdam Amsterdam Netherlands NL-1018 TV V Amsterdam, Netherlands
Titolo Testata:
PHYTOCHEMISTRY
fascicolo: 1, volume: 54, anno: 2000,
pagine: 5 - 11
SICI:
0031-9422(200005)54:1<5:PACOVH>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEAWEED ASCOPHYLLUM-NODOSUM; DEPENDENT HALOPEROXIDASES; CORALLINA-PILULIFERA; BROMOPEROXIDASE; PHAEOPHYCEAE; PEROXIDASES;
Keywords:
Pelvetia canaliculata; Fucaceae; vanadium-dependent haloperoxidases; iodoperoxidases; vanadium in biology;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: da Silva, JJRF Inst Super Tecn, Ctr Quim Estrutural, Complexo 1,Av RoviscoPais 1, P-1049001 Lisbon, Portugal Inst Super Tecn Complexo 1,Av Rovisco Pais 1 Lisbon Portugal P-1049001
Citazione:
M.G. Almeida et al., "Purification and characterisation of vanadium haloperoxidases from the brown alga Pelvetia canaliculata", PHYTOCHEM, 54(1), 2000, pp. 5-11

Abstract

Two enzymes characterised as iodoperoxidases (PcI and PcII), with vanadium-dependent activity, have been purified from the brown alga Pelvetia canaliculata (L. ) Decne et Thur. (Fucaceae, Phaeophyceae), collected in the Northern Portuguese coast, at Viana do Castelo. The relative molecular masses were 166 kDa for PcI and 416 kDa for PcII, as determined by gel filtration. SDS-PAGE shows that PcI has just one band corresponding to a subunit of 66 kDa, while PcII shows four bands (66, 72, 157 and 280 kDa). The following kinetic parameters have been determined from a steady-state analysis of the oxidation of iodide by H2O2: PcI, pH(opt) = 6.0, K-M(I-) = 2.1 mM, K-M(H2O2)= 110 mu M, K-i(I-) = 127 mM, and PcII, pH(opt) = 6.5, K-M(I-) = 2.4 mM, K-M(H2O2) = 20 mu M and k(i)(I-) = 69 mM. These iodoperoxidases are thermostable, as also observed for vanadium bromo- and chloroperoxidases. (C) 2000 Elsevier Science ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 01:18:41