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Titolo:
Reverse genetics in zebrafish
Autore:
Lekven, AC; Helde, KA; Thorpe, CJ; Rooke, R; Moon, RT;
Indirizzi:
Univ Washington, Sch Med, Ctr Dev Biol, Dept Pharmacol,Howard Hughes Med Inst, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 ghes Med Inst, Seattle, WA 98195 USA
Titolo Testata:
PHYSIOLOGICAL GENOMICS
fascicolo: 2, volume: 2, anno: 2000,
pagine: 37 - 48
SICI:
1094-8341(20000313)2:2<37:RGIZ>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
ETHYL-N-NITROSOUREA; GERM-CELLS; CAENORHABDITIS-ELEGANS; TRANSGENIC ZEBRAFISH; SKELETAL-MUSCLE; DANIO-RERIO; LINKAGE MAP; MUTATIONS; MUTAGENESIS; IDENTIFICATION;
Keywords:
mutagenesis; trimethylpsoralen; gamma rays; ethylnitrosourea; functional genomics;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Lekven, AC Univ Washington, Sch Med, Ctr Dev Biol, Dept Pharmacol,Howard Hughes Med Inst, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195st, Seattle, WA 98195 USA
Citazione:
A.C. Lekven et al., "Reverse genetics in zebrafish", PHYSIOL GEN, 2(2), 2000, pp. 37-48

Abstract

The zebrafish has become a popular model system for the study of vertebrate developmental biology because of its numerous strengths as a molecular genetic and embryological system. To determine the requirement for specific genes during embryogenesis, it is necessary to generate organisms carrying loss-of-function mutations. This can be accomplished in zebrafish through a reverse genetic approach. This review discusses the current techniques for generating mutations in known genes in zebrafish. These techniques include the generation of chromosomal deletions and the subsequent identification of complementation groups within deletions through non-complementation assays. In addition, this review will discuss methods currently being evaluated that may improve the methods for finding mutations in a known sequence, including screening for randomly induced small deletions within genes and screening for randomly induced point mutations within specific genes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 06:17:00