Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Ultrastructural evidence of the protective effect of Na+/H+ exchange inhibition on the in vitro damage induced by ischaemia reperfusion in the interventricular septum of the rabbit heart
Autore:
Salinas, P; Gil-Loyzaga, P; Barrigon, S;
Indirizzi:
Univ Complutense, Sch Med, Dept Pharmacol, E-28040 Madrid, Spain Univ Complutense Madrid Spain E-28040 t Pharmacol, E-28040 Madrid, Spain Univ Complutense, Cell Culture Ctr, E-28040 Madrid, Spain Univ Complutense Madrid Spain E-28040 Culture Ctr, E-28040 Madrid, Spain
Titolo Testata:
PHARMACOLOGY & TOXICOLOGY
fascicolo: 5, volume: 86, anno: 2000,
pagine: 222 - 227
SICI:
0901-9928(200005)86:5<222:UEOTPE>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCED MYOCARDIAL NECROSIS; CONTRACTION BAND LESIONS; GUINEA-PIG HEARTS; DIMETHYL AMILORIDE; INTRACELLULAR PH; ISCHEMIC-INJURY; NA/H EXCHANGE; INFARCT SIZE; H+ EXCHANGE; RAT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Barrigon, S Univ Complutense, Sch Med, Dept Pharmacol, Avda Complutense S-N, E-28040 Madrid, Spain Univ Complutense Avda Complutense S-N Madrid Spain E-28040 in
Citazione:
P. Salinas et al., "Ultrastructural evidence of the protective effect of Na+/H+ exchange inhibition on the in vitro damage induced by ischaemia reperfusion in the interventricular septum of the rabbit heart", PHARM TOX, 86(5), 2000, pp. 222-227

Abstract

We investigated the effects of the Na+/H+ antiporter inhibitor, dimethylamiloride, on myocardial injury after I h global ischaemia and 30 min. reperfusion in the isolated arterially perfused interventricular septum of the rabbit heart. After ischaemia and reperfusion challenge, dimethylamiloride significantly increased the recovery of developed tension in a dose-dependentmanner, and significantly decreased the maximal increase in resting tension. Ultrastructural analysis of myocytes submitted to the experimental in vitro model supported functional maintenance of physiologically-like conditions. Where myocardial portions were submitted to ischaemic conditions and reperfusion, myocyte cell damage reached usual characteristics of infarct-like induced lesions. Intracellular oedema, severe disruption of myofibrils with loss of muscle striation and both swelling and fragmentation of mitochondria were the main characteristics observed. Dimethylamiloride treatment clearly modifies ultrastructural findings towards the normalization of cell shape and structure, only a slight-middle intracellular oedema and contraction bands were found. On the basis of the present results, we suggest that the protective effects exhibited by dimethylamiloride on the ischaemic myocardium are compatible with its Na+/ H+ antiporter inhibition properties, they diminish Na+ accumulation and then either Ca2+ overload or non-exocytoticnoradrenaline release during the ischaemia and reperfusion challenge.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 09:24:35